MicroRNAs (miRNAs) are gene expression regulators that play an important role in drug addiction. We previously reported miR-204-3p was the only up-regulated miRNA in the nucleus accumbens (NAc) in methamphetamine (METH)-sensitized mice. In this study, we are reporting a miR-204-3p potential mechanism in METH sensitization. We first measured the expression changes of miR-204-3p in the NAc of METH- sensitized mice. Then we predicted the targets of miR-204-3p by bioinformatics tools and combined the potential targets with the METH-responsive genes from the ArrayExpress database. KEGG pathway analyses were performed to investigate the prospective mechanisms and four enriched genes were validated by RT-PCR. As a result, miR-204-3p showed a shift from down-regulation to up-regulation in the NAc from the development to the expression of METH sensitization. Bioinformatics analysis predicted 1834 putative targets, 259 of which were differentially expressed in the NAc in response to METH. These targets were significantly enriched in axon guidance (P = 9.59 × 10). Four putative targets (Sema3A, Plxna4, Rac1, and Pak3) enriched in axon guidance also exhibited significant changes in the NAc after METH challenge injection. Moreover, expression levels of miR-204-3p, Sema3A and Plxna4 exhibited a negative association in the expression of METH sensitization. It appeared that miR-204-3p may be involved in the expression of METH sensitization by regulating the expression of Sema3A and Plxna4. Our study provided a potential network of miR-204-3p-axon guidance in the NAc in the expression of METH-induced behavioral sensitization.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.neulet.2019.134303 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Theta and gamma modulation in the nucleus accumbens as drivers of neurophysiological responses to acute methamphetamine sensitization in mice.
Exp Brain Res
November 2024
Division of Health and Applied Science Physiology Program, Faculty of Science, Prince of Songkla University, Hat Yai, Thailand.
Methamphetamine (METH) has well-documented long-term effects on the brain, including increased psychomotor activity and behavioral sensitization. However, its immediate effects on the brain's reward system following acute exposure, which may contribute to the development of addiction, are less understood. This study aimed to investigate the effects of acute METH on brain oscillations in the nucleus accumbens of C57BL/6 mice.
View Article and Find Full Text PDFIs the Use of the Extended (Meth)acrylate Series - Nails Justified? Characterization of Nail Acrylate Allergy in a Tertiary Medical Centre.
Acta Derm Venereol
November 2024
Department of Dermatology, Rambam Health Care Campus, Haifa, Israel; The Ruth and Bruce Rappaport Faculty of Medicine, Technion - Israel Institute of Technology, Haifa, Israel.
Article Synopsis
- Methacrylate allergy, linked to allergic contact dermatitis, has become more prevalent, leading to the addition of 2-hydroxyethyl methacrylate to the European Baseline Series of allergens.
- A study analyzing 3,828 patients showed that 396 were tested for various methacrylate allergens, with 38.6% testing positive for at least one, primarily 2-hydroxyethyl methacrylate.
- The research indicated that including additional methacrylates, like hydroxypropyl methacrylate and ethylene glycol dimethacrylate, improved detection rates significantly, suggesting that broader testing could identify more cases of allergy.
Dopamine D3 receptor mediates natural and methamphetamine rewards via regulating the expression of miR-29c in the nucleus accumbens of mice.
Neuropharmacology
January 2025
College of Forensic Medicine, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, 710061, PR China; The Key Laboratory of Health Ministry for Forensic Science, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, PR China; Institute of Drug Dependence and Neuroscience, Bio-Evidence Sciences Academy, Western China Science and Technology Innovation Harbor, Xi'an Jiaotong University, Xi'an, Shaanxi, PR China. Electronic address:
The dopamine D3 receptor (D3R), principally confined to the nucleus accumbens (NAc), is involved in regulating natural and drug rewards; however, the molecular mechanisms underlying the associated process remain unclear. Earlier research has reported the concurrent influence of D3R and miR-29c expressed in the NAc on methamphetamine (METH)-induced reward behaviors and microglial activation, hinting at regulatory roles in reward processing. Herein, we performed viral manipulation-mediating D3R/miR-29c overexpression and inhibition in the whole NAc in male D3R knockout and wild-type mice to investigate this potential relationship.
View Article and Find Full Text PDFMethamphetamine-Induced Blood Pressure Sensitization Correlates with Morphological Alterations within A1/C1 Catecholamine Neurons.
Int J Mol Sci
September 2024
Istituto di Ricovero e Cura a Carattere Scientifico (IRCCS) Neuromed, 86077 Pozzilli, Italy.
Methamphetamine (METH) is a drug of abuse, which induces behavioral sensitization following repeated doses. Since METH alters blood pressure, in the present study we assessed whether systolic and diastolic blood pressure (SBP and DBP, respectively) are sensitized as well. In this context, we investigated whether alterations develop within A1/C1 neurons in the vasomotor center.
View Article and Find Full Text PDFMelatonin Regulates Neuronal Synaptic Plasticity in the Supramammillary Nucleus and Attenuates Methamphetamine-Induced Conditioned Place Preference and Sensitization in Mice.
J Pineal Res
September 2024
Department of Anatomy and Neurobiology, Shandong University School of Basic Medicine, Jinan, Shandong, China.
Article Synopsis
- Methamphetamine (METH) negatively impacts health and affects memory systems, particularly through the supramammillary nucleus (SuM), which is involved in spatial and contextual memory.
- Melatonin (MLT) appears to counteract some effects of METH, as previous studies indicated it can reduce METH-induced locomotor sensitization.
- The current study shows that MLT lowers certain neural responses and addictive behaviors induced by METH in a mouse model, suggesting that MLT may help regulate synaptic plasticity in the SuM via its specific receptors.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!