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Enhancing trace DNA profile recovery in forensic casework using the amplicon RX post-PCR clean-up kit.
Sci Rep
January 2025
The Biology and DNA Section, General Department of Forensic Science and Criminology, Dubai Police General Head Quarters, Dubai, United Arab Emirates.
This study evaluated the effectiveness of the amplicon RX post-PCR clean-up kit in enhancing trace DNA profile recovery from forensic casework samples amplified using the GlobalFiler PCR amplification kit. The impact of post-PCR clean-up on allele recovery and signal intensity was assessed in both trace casework samples and control samples across a range of DNA concentrations. The results showed that the amplicon RX method significantly improved allele recovery compared to the 29-cycle protocol (p = 8.
View Article and Find Full Text PDFMethod development and validation for the simultaneous determination of 21 antiviral drugs by ultra-high performance liquid chromatography-tandem mass spectrometry in chicken muscle and liver.
J Chromatogr A
January 2025
Wageningen Food Safety Research (WFSR), European Union Reference Laboratory for antivirals, growth promotors and sedatives, Part of Wageningen University & Research, Wageningen 6700 AE, the Netherlands. Electronic address:
The recent unauthorization of antiviral drugs in food-producing animals according to Commission Delegated Regulation (EU) 2022/1644 have increased the need for food control laboratories to develop analytical methods and perform official controls. In this work, a simple and fast analytical methodology was developed for the simultaneous determination of 21 antiviral drugs in chicken muscle and liver by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). Chromatographic separation was achieved by an HILIC BEH amide column; followed by detection with a electrospray ionization source in positive and negative modes.
View Article and Find Full Text PDFQuantification and profiling of urine cells by integrated cytocentrifugation and infrared spectroscopy.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
Department of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100 Burjassot, Spain. Electronic address:
The presence of cells in urine and in particular White Blood Cells (WBCs) is often associated with Urinary Tract Infections (UTIs) and other diseases. Non-invasive screening of WBCs requires the development of cost-effective point of care diagnostic tools. Infrared (IR) spectroscopy has the potential to identify and quantify cells in urine.
View Article and Find Full Text PDFHighly efficient and convenient QuEChERS using ZIF-67 derived magnetic nanoporous carbon for determination of carbamate pesticides in various vegetable and fruit samples.
Food Chem
January 2025
School of Public Health, Hebei Key Laboratory of Occupational Health and Safety for Coal Industry, North China University of Science and Technology, Tangshan 063210, Hebei, China. Electronic address:
Effective and convenient QuEChERS of lipophilic pesticides with wide pK range from strongly pigment-rich food samples remains a great challenge. Here, a ZIF-67 derived magnetic nanoporous carbon (Co@MPC) was firstly proposed for modified QuEChERS of carbamate pesticides (pK 4.3-12.
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