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Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency. | LitMetric

Cell cycle dynamics of mouse embryonic stem cells in the ground state and during transition to formative pluripotency.

Sci Rep

Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Instituto de Química Biológica (IQUIBICEN), Laboratorio de Regulación Génica en Células Madre, Buenos Aires, Argentina.

Published: May 2019

AI Article Synopsis

Article Abstract

Mouse embryonic stem cells (mESCs) can be maintained as homogeneous populations in the ground state of pluripotency. Release from this state in minimal conditions allows to obtain cells that resemble those of the early post-implantation epiblast, providing an important developmental model to study cell identity transitions. However, the cell cycle dynamics of mESCs in the ground state and during its dissolution have not been extensively studied. By performing live imaging experiments of mESCs bearing cell cycle reporters, we show here that cells in the pluripotent ground state display a cell cycle structure comparable to the reported for mESCs in serum-based media. Upon release from self-renewal, the cell cycle is rapidly accelerated by a reduction in the length of the G1 phase and of the S/G2/M phases, causing an increased proliferation rate. Analysis of cell lineages indicates that cell cycle variables of sister cells are highly correlated, suggesting the existence of inherited cell cycle regulators from the parental cell. Together with a major morphological reconfiguration upon differentiation, our findings support a correlation between this in vitro model and early embryonic events.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6541595PMC
http://dx.doi.org/10.1038/s41598-019-44537-0DOI Listing

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