The influence of temperature on growth and verotoxin production by Escherichia coli strains was studied in brain heart infusion (BHI) broth both in shake cultures at various temperatures and in a temperature-gradient incubator. All strains of E. coli surveyed grew from at least 10 to 45°C, with some strains growing at 8° C. Verotoxin production (determined using the Vero cell-assay system) was a function of both temperature and time, with the highest titers produced at temperatures supporting the fastest growth (based on days to visible turbidity) and highest viable cell counts. However, for strains producing verotoxin, toxin production was detected at any temperature supporting growth. Three strains (of 16 tested) increased 1000-fold in viable count in 4 to 6 days at 10°C. The data presented here indicate that most E. coli strains surveyed can easily grow at ca. 10°C and thus suggest the potential for growth in temperature-abused refrigerated foods.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4315/0362-028X-58.4.352 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Shiga Toxin: Emerging Producer Strains, Prophylactic Approaches, and Application in Cancer Therapy.
J Cancer Prev
December 2024
Infectious Disease Research Center, Avicenna Institute of Clinical Sciences, Hamadan, IranAvicenna Health Research Institute, Hamadan University of Medical Sciences, Hamadan, Iran.
Shiga toxin-producing is the most prevalent bacterial strain responsible for Shiga toxin-related infections. While Shiga toxin is inherently toxic, it has potential therapeutic applications as a component of anticancer drugs. Despite its association with infections and harmful effects on human health, Shiga toxin is being explored as a viable element in drug delivery systems targeting cancer cells.
View Article and Find Full Text PDFShiga Toxins Produced by Enterohaemorrhagic Induce Inflammation in Toxin-Sensitive Cells through the p38 MAPK/MK2/Tristetraprolin Signaling Pathway.
J Microbiol Biotechnol
December 2024
Environmental Diseases Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
Shiga toxins (Stxs), produced by serotype 1 and certain pathotypes, cause hemorrhagic colitis, which can progress to hemolytic uremic syndrome (HUS) and central nervous system (CNS) pathology. The underlying mechanisms of toxin-induced inflammation remain unclear. The p38 mitogen-activated protein kinase (MAPK) and its downstream target, MAPKAPK2 (MK2), play key roles in various cellular responses.
View Article and Find Full Text PDFHuman Gb3/CD77 synthase: a glycosyltransferase at the crossroads of immunohematology, toxicology, and cancer research.
Cell Mol Biol Lett
November 2024
Laboratory of Glycobiology, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla 12, 53-114, Wroclaw, Poland.
Article Synopsis
- Human Gb3/CD77 synthase, an enzyme, creates Galα1→4Gal structures on glycosphingolipids and glycoproteins, which are important for bacterial recognition in infections.
- The major product, Globotriaosylceramide (Gb3), acts as a receptor for harmful toxins from certain bacteria and is also linked to symptoms of Anderson-Fabry disease due to enzyme deficiency.
- Additionally, the synthase is implicated in cancer biology, playing a role in cancer cell survival and resistance to treatments, and influencing the P1PK blood group system.
Antimicrobial Resistance in Diverse Pathotypes from Nigeria.
Antibiotics (Basel)
September 2024
School of Health and Life Sciences, Teesside University, Middlesbrough TS1 3BX, UK.
is a gram-negative commensal bacterium living in human and animal intestines. Its pathogenic strains lead to high morbidity and mortality, which can adversely affect people by causing urinary tract infections, food poisoning, septic shock, or meningitis. Humans can contract by eating contaminated food-such as raw or undercooked raw milk, meat products, and fresh produce sold in open markets-as well as by coming into contact with contaminated settings like wastewater, municipal water, soil, and faeces.
View Article and Find Full Text PDFField efficacy of a recombinant toxoid vaccine against Shiga toxin 2e during a naturally occurring edema disease infection.
Can J Vet Res
October 2024
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
The objective of this field trial was to determine the efficacy of a recombinant toxoid vaccine against Shiga toxin 2e (Stx2e) in piglets suffering from edema disease (ED). Three farms with confirmed ED cases were selected for the field trials. On each farm, a total of 40 4-day-old pigs were randomly allocated to either the vaccinated or unvaccinated group, with 20 pigs per group (10 males and 10 females).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!