Autotrophic nitrogen removal in an integrated fixed-biofilm activated sludge (IFAS) reactor: Anammox bacteria enriched in the flocs have been overlooked.

In this study, an autotrophic nitrogen removal process was established using an integrated fixed-biofilm activated sludge (IFAS) reactor treated with high ammonium wastewater. A nitrogen removal rate (NRR) of 2.78 kg N/(m·d) was obtained during the 206-day operation. Moreover, during the stable period, the large flocs (D > 0.2 mm) had a significantly higher abundance of anammox bacteria than the small flocs (D < 0.2 mm) and biofilm, resulting in 51% of the anammox bacteria being located in the flocs. The result indicates that anammox bacteria can be enriched in the flocs and in the biofilm, which has been rarely reported for IFAS reactors. In addition, the large flocs are likely formed through biofilm detachment since the microbial community was similar for the two kinds of biomass. Overall, the role of flocs in IFAS reactors are complicated and their contribution to the anammox reaction have been overlooked thus far.