One of the fundamental aspects in understanding the biology, diversity and epidemiology of a parasite lies in its proper identification. In the present study, morphological and molecular characterization of Clinostomum metacercariae recovered from an ornamental fish, Trichogaster fasciata, was carried out in order to ascertain its identity. To serve the purpose, scanning electron micrographs and gene sequences for two commonly used molecular markers, i.e., nuclear ribosomal internal transcribed spacer 2 (rDNA-ITS2) and mitochondrial cytochrome c oxidase subunit 1 (mtCO1) were obtained. The sequences were further used for generating similarity index matrix as well as inferring phylogenies. Light and electron microscopic observations on metacercariae of the parasite revealed that it belongs to the genus Clinostomum. Identification of the same up to the level of species was made possible through sequence and phylogenetic analyses. The ITS2 sequence analyses of our species (KX758630) showed similarity to unidentified Clinostomum sp. reported from Nigeria (KY865625) and China (KP110579), and C. tilapiae recorded from South Africa (KX034048) and Nigeria (KY649353). However, the CO1 gene analyses suggested it to be highly identical to C. philippinense and the same was also corroborated in the phylogenetic analysis. Thus, morphological and molecular characterization revealed that the recovered metacercariae belong to the species C. philippinense. Additionally, a brief description of secondary structures of ITS2 of various species of Clinostomum has also been discussed.
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http://dx.doi.org/10.17420/ap6501.187 | DOI Listing |
Acta Parasitol
January 2025
Department of Parasitology, Faculty of Veterinary Medicine, Ondokuz Mayıs University, Samsun, Turkey.
Purpose: The aim of this study was to investigate the presence of Clinostomum species in wild birds in Turkey using morphological and molecular methods.
Methods: 51 birds of 18 species from seven orders previously reported as definitive hosts of the Clinostomum spp. were collected.
Vet Med Int
November 2024
Parasitology Unit, Department of Pathology and Biomarkers in Animal Parasitology Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.
J Helminthol
November 2024
Killifish Foundation, La Plata, Buenos Aires Province, Argentina.
Clinostomids are a group of digeneans in which substantial diversity has been recently discovered, with some metacercariae specific to their fish hosts. Genetic analysis has been instrumental in elucidating species diversity within this genus. Recently, seven COI lineages were reported in Argentina, along with three metacercarial morphotypes lacking molecular data.
View Article and Find Full Text PDFSci Rep
October 2024
Parasitology Department, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Fish-borne zoonotic trematodes (FBZTs) pose significant health risks and economic challenges worldwide. This study investigated the prevalence of encysted metacercariae (EMCs) in Nile tilapia (Oreochromis niloticus) from two Egyptian governorates and evaluated the antiparasitic efficacy of chitosan, silver, and selenium nanoparticles against these parasites. A cross-sectional analysis of 453 O.
View Article and Find Full Text PDFBMC Vet Res
October 2024
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
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