AI Article Synopsis
- Meiosis is a specialized type of cell division that produces gametes and involves meiotic recombination, which creates genetic diversity and ensures proper chromosome separation.
- DMC1 is a key protein that assists in pairing homologous chromosomes during this process by allowing DNA strands to exchange genetic material.
- The chapter details an in vitro assay to study DMC1's role in DNA strand exchange over long stretches of DNA, helping in understanding its function alongside other proteins involved in homologous recombination.
Article Abstract
Meiosis is a specialized cell division that generates gametes. Meiotic recombination is essential not only to generate diversity in offspring, but also to hold homologous chromosomes together through chiasma allowing proper chromosome segregation. This process requires the meiosis-specific recombinase, DMC1. DMC1 facilitates the search for homology between the homologous chromosomes and is followed by DNA strand invasion and strand exchange to produce a linkage between the two homologous chromosomes. The development of biochemical in vitro assays and the purification of the requisite proteins factors has led to a better understanding of the molecular mechanisms of meiotic homologous recombination. In this chapter, a detailed in vitro assay to examine DNA strand exchange over 5000 bases of DNA catalyzed by human DMC1 is described. This method has proved to be valuable for examining the catalytic potential of hDMC1 and delineating the functional interaction with other HR factors.
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| http://dx.doi.org/10.1007/978-1-4939-9500-4_17 | DOI Listing |
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