A complementation assay was developed to determine whether alleles of DNA repair genes are necessary for repairing specific types of damage. The assay was established by measuring the resistance capacity of Rad51d-deficient mouse embryonic fibroblasts (MEFs) transfected with mammalian expression constructs. Here, we describe the methods used to assess colony survival following the treatment of transfected cells with genotoxic compounds. This approach provides a time-efficient and stringent strategy to screen genetic alleles for identifying regions or specific amino acid residues critical for function or regulation of DNA repair pathways.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-9500-4_12 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A Series of Novel Alleles of Modulating Heading and Salt Tolerance in Rice.
Plants (Basel)
January 2025
State Key Laboratory of Rice Biology and Breeding, China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 311400, China.
Rice ( L.) is a staple crop for nearly half of the global population and one of China's most extensively cultivated cereals. Heading date, a critical agronomic trait, determines the regional and seasonal adaptability of rice varieties.
View Article and Find Full Text PDFThe Potassium Utilization Gene Network in and Functional Validation of Gene in Response to Potassium Deficiency.
Int J Mol Sci
January 2025
College of Agronomy and Biotechnology, Southwest University, Chongqing 400716, China.
Potassium, an essential inorganic cation, is crucial for the growth of oil crops like L. Given the scarcity of potassium in soil, enhancing rapeseed's potassium utilization efficiency is of significant importance. This study identified 376 potassium utilization genes in the genome of ZS11 through homologous retrieval, encompassing 7 functional and 12 regulatory gene families.
View Article and Find Full Text PDFThe Passage of Chaperonins to Extracellular Locations in Requires a Functional Dot/Icm System.
Biomolecules
January 2025
Department of Microbiology-Immunology, Faculty of Medicine, Dalhousie University, Halifax, NS B3H 4R2, Canada.
HtpB, the chaperonin of the bacterial pathogen , is found in extracellular locations, even the cytoplasm of host cells. Although chaperonins have an essential cytoplasmic function in protein folding, HtpB exits the cytoplasm to perform extracellular virulence-related functions that support 's lifestyle. The mechanism by which HtpB reaches extracellular locations is not currently understood.
View Article and Find Full Text PDFLung seven transmembrane receptors are involved in Arabidopsis root growth mediated by Danger-associated peptide Pep1.
Biochem Biophys Res Commun
January 2025
State Key Laboratory for Pharmaceutical Biotechnology, College of Life Sciences, Nanjing University, 210093, Nanjing, China.
Lung seven transmembrane receptor family is a small part of Arabidopsis gene family. So far, the function of some members of the this family is unknown. Plant elicitor peptide1 (Pep1) is one of damage-associated molecular patterns (DAMPs), which could trigger root growth inhibition and plant immunity responses.
View Article and Find Full Text PDFComprehensive genomic analysis of SmbHLH genes and the role of SmbHLH93 in eggplant anthocyanin biosynthesis.
Plant Cell Rep
January 2025
School of Life Science, Anhui Agricultural University, Hefei, 230036, China.
SmbHLH93can activate the expression of SmCHS, SmANS, SmDFR and SmF3H.Overexpression of SmbHLH93promotes anthocyanin biosynthesis. SmbHLH93can interact with SmMYB1 to promote anthocyanin accumulation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!