Detection of Genotypes Among Birds in Northeast Iran.

We determined the prevalence of genotypes in asymptomatic and symptomatic birds in northeast Iran. Samples were collected from 11 species of Psittaciformes and 1 species of Columbiformes from 2015 to 2016. Choanal cleft and cloacal swab samples, fresh fecal samples, and/or tissue samples of 70 symptomatic and 130 asymptomatic birds were collected and tested by molecular detection (nested polymerase chain reaction [PCR] testing specific for ). Results showed was detected in 37 (18.5%) of 200 birds (18/37 symptomatic and 19/37 asymptomatic birds) by nested PCR assay. Of the PCR-positive samples, 14 products were positive for oligonucleotide sets CTU/CTL by a second PCR assay and genotyped by outer membrane protein A (A) gene sequencing. Of the 10 samples positive for genotype A (cockatiels [, n = 5], ring-necked parakeet [, n = 2], African gray parrot [, n = 3]), 6 samples were from asymptomatic and 4 from symptomatic birds. Genotype B was observed in 3 samples from symptomatic birds ( [n = 2], pigeon [, n = 1]), and provisional genotype I was detected in one symptomatic cockatiel. These findings revealed the importance of monitoring imported asymptomatic birds in developing countries, especially the Middle East, where there is no systematic monitoring. To the best of our knowledge, this is the first report regarding the detection of provisional genotype I in cockatiels.