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Determination of oligonucleotide deamination by high resolution mass spectrometry. | LitMetric

Determination of oligonucleotide deamination by high resolution mass spectrometry.

J Pharm Biomed Anal

Ionis Pharmaceuticals, Carlsbad, CA 92010, United States. Electronic address:

Published: September 2019

AI Article Synopsis

Article Abstract

A novel analytical approach capable of measuring deaminated degradation products of oligonucleotide therapeutics is described. The method employs high-resolution mass spectrometry to assess the shift in isotopic distribution that accompanies deamination. Isotopic Distribution Factors (IDF), derived directly from the peak heights of the isotopic pattern, are employed to measure deamination levels of as little as 0.5%. Results obtained from application of the method to a phosphorothioate diester oligonucleotide exposed to various temperatures were used to determine deamination rates.

Download full-text PDF

Source
http://dx.doi.org/10.1016/j.jpba.2019.05.012DOI Listing

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