The study aimed to evaluate the histopathological characteristics of renal lesions in chronically infected sheep and with low titers of anti-Leptospira antibodies from a slaughterhouse. In the serological analysis, 24.74% (48/194) presented seroreactivity with a titer equal to or greater than 100. Among these seroreactive sheep, titers of 100 were predominant (58.33%, 28/48), with the highest titer being 1,600 (2.08%, 1/48). Serogroup Sejroe (sv. Hardjo) was the most frequent at 35.42% (17/48). Leptospiral DNA was verified in 4.12% (8/194) of the kidney samples tested, and no urine sample was positive. All the samples corresponded to the pathogenic species L. interrogans. The eight amplicons with 202-nucleotides were identical with two mismatches (presented 100% of identity) using the PCR targeting to secY gene. Histological sections of PCR-positive kidneys were submitted to direct detection by the anti-LipL32 immunohistochemistry (IHC) technique. The Leptospira spp. antigen was evident in 62.5% (5/8) of the kidneys. Positive staining was observed in the cytoplasm of tubular cells and in the form of brownish aggregates that adhered to tubular epithelial cells and projected into the lumen. Inflammatory lymphoplasmacytic infiltrate, ranging from mild to moderate, with multifocal distribution, was the predominant finding in seroreactive animals (33.33%, 16/48). The demonstration of the leptospiral antigen lining the renal tubules through IHC of naturally infected sheep confirmed by PCR characterizes renal colonization in a species with the presence of histological changes compatible with leptospirosis.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6532964 | PMC |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0217391 | PLOS |
Curr Res Microb Sci
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Kidney Research Center, Chang Gung Memorial Hospital, Linkou,333, Taiwan.
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Department of Microbiology, Virology, and Immunology, I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine.
Leptospirosis, a widespread zoonotic disease caused by spp., affects approximately 1 million people annually and causes about 58,000 deaths worldwide. This study examines the epidemiology of leptospirosis in Ukraine from 2018 to 2023, focusing on the impact of weather and geographical factors on disease transmission.
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Laboratório de Desenvolvimento de Vacinas, Instituto Butantan, São Paulo, Brazil.
Pathogenic Leptospira is the etiological cause of the zoonotic life-threatening infection called leptospirosis. The disease is spread worldwide with higher risk in tropical regions. Although leptospirosis represents a burden to the health of humans and animals, the pathogenic mechanisms of Leptospira infection are yet to be clarified.
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Grupo de Enfermedades Infecciosas, Departamento de Microbiología, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, Colombia.
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Universidade Federal de Santa Maria (UFSM), Centro de Ciências Rurais (CCR), Departamento de Medicina Veterinária Preventiva (DMVP), Programa de Pós-graduação em Medicina Veterinária (PPGMV), Santa Maria, Rio Grande do Sul CEP 97105-900, Brazil; Universidade Federal de Santa Maria (UFSM), Centro de Ciências da Saúde (CCS), Departamento de Microbiologia e Parasitologia (DMIP), Laboratório de Diagnóstico e Pesquisa em Leptospirose (LabLepto), Santa Maria CEP 97105-900, Brazil. Electronic address:
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