We studied the production of PGE2 by human conjunctival and corneal cells in response to inflammation, and reduction of inflammation with non-steroidal anti-inflammatory drugs. Primary cultures of human conjunctival epithelial cells, fibroblasts, corneal epithelial cells, and keratocytes were incubated with IL-4 and TNF-α. PGE2 and COX-2 levels were analyzed. Effects of anti-inflammatory and anti-immune drugs on PGE2 production were also investigated. IL-4 and TNF-α induced the generation of PGE2 and COX-2 in conjunctival and corneal cells. Epithelial PGE2 production was significantly lower than in keratocytes and fibroblasts, which was down-regulated by aspirin. IL-4 and TNF-α enhanced the inflammatory response via prostaglandin production which contributed to ocular surface inflammation. Prostaglandin production was higher in stromal cells than epithelial cells. These results suggest that the epithelial barrier disruption may contribute to ocular allergic inflammation by the PGE2 production from stromal cells. Moreover, NSAIDs were effective in suppressing PGE2 production in our experiment.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1080/09273948.2019.1595669 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Inducing phospholipase A2 and cyclooxygenase-2 expression and prostaglandins' production of human dental pulp cells by activation of NOD receptor and its downstream signaling.
Int J Biol Macromol
December 2024
School of Dentistry, National Taiwan University Medical College, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, Taipei, Taiwan; School of Dentistry, College of Dental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Department of Dentistry, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan. Electronic address:
Dental caries with invasion and infection by microorganisms may induce pulpitis and intolerable pain. L-Ala-γ-D-Glu-mDAP (TriDAP) is a DAP-comprising muramyl tripeptide and a peptidoglycan degradation product found in gram-negative pulpal pathogens. TriDAP activates nucleotide-binding oligomerization domain1/2 (NOD1/NOD2) and induces tissue inflammatory responses.
View Article and Find Full Text PDFProstanoid signaling in retinal cells elicits inflammatory responses relevant to early-stage diabetic retinopathy.
J Neuroinflammation
December 2024
Department of Pharmacology, Vanderbilt University, Nashville, TN, USA.
Inflammation is a critical driver of the early stages of diabetic retinopathy (DR) and offers an opportunity for therapeutic intervention before irreversible damage and vision loss associated with later stages of DR ensue. Nonsteroidal anti-inflammatory drugs (NSAIDs) have shown mixed efficacy in slowing early DR progression, notably including severe adverse side effects likely due to their nonselective inhibition of all downstream signaling intermediates. In this study, we investigated the role of prostanoids, the downstream signaling lipids whose production is inhibited by NSAIDs, in promoting inflammation relevant to early-stage DR in two human retinal cell types: Müller glia and retinal microvascular endothelial cells.
View Article and Find Full Text PDFEffect of a dietary nutraceutical "STRUCTURE-Joint" on response of horses to intra-articular challenge with IL-1: implications for tissue adaptation to stress.
Transl Anim Sci
December 2024
Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
The purpose was to determine local (articular) and systemic effects of intra-articular interleukin-1 in horses supplemented with a dietary PUFA supplement [STRUCTURE-Joint ()]. Sixteen (16) healthy, mature, light breed horses were randomly assigned to diets containing 0 or 120 mL ( = 8 per group) of ST-J for 30 d. On days 0 (prior to beginning supplementation) and 27, recombinant equine interleukin-1β () (75 ng) was injected into the left or right intercarpal joint to induce mild, transient synovitis.
View Article and Find Full Text PDFCervical ripening at home or in hospital during induction of labour: the CHOICE prospective cohort study, process evaluation and economic analysis.
Health Technol Assess
December 2024
Usher Institute, University of Edinburgh, Edinburgh, UK.
Background: Around one in three pregnant women undergoes induction of labour in the United Kingdom, usually preceded by in-hospital cervical ripening to soften and open the cervix.
Objectives: This study set out to determine whether cervical ripening at home is within an acceptable safety margin of cervical ripening in hospital, is effective, acceptable and cost-effective from both National Health Service and service user perspectives.
Design: The CHOICE study comprised a prospective multicentre observational cohort study using routinely collected data (CHOICE cohort), a process evaluation comprising a survey and nested case studies (qCHOICE) and a cost-effectiveness analysis.
[Protective effect of Codonopsis Radix fermented with Lactobacillus plantarum on gastric mucosa in rat model of acute gastric ulcer].
Zhongguo Zhong Yao Za Zhi
September 2024
School of Life Sciences, Lanzhou University Lanzhou 730000, China.
This study aims to decipher the mechanism of the broth of Codonopsis Radix fermented with Lactobacillus plantarum(FCR) on the gastric mucosa in the rat model of acute gastric ulcer. A total of 64 SD rats were randomized into 8 groups: control, model, positive control(omeprazole), probiotic, unfermented Codonopsis Radix, and low-, medium-and high-dose FCR, with 8 rats in each group. The rat model of acute gastric ulcer was established by gavage with anhydrous ethanol.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!