AI Article Synopsis
- The study used advanced mass spectrometry methods to analyze the effects of grafting different rootstocks on non-volatile metabolites in tea leaves, focusing on one non-grafted strain and four grafted varieties.
- A total of 32 different metabolites were identified, including various phenolic compounds and flavonoids, with significant differences based on the rootstock used, particularly noting exceptional metabolic regulation by WLH and BY rootstocks.
- The findings highlight three essential metabolic pathways influenced by grafting, which could potentially impact tea quality, providing a scientific foundation for understanding how grafting affects tea leaf composition.
Article Abstract
To investigate the effects of grafting on non-volatile metabolites in tea, non-targeted metabolomic analyses of fresh leaves were performed on the basis of ultra-high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF/MS). One non-grafted YingHong No. 9 and four grafted tea [grafting scion YingHong No. 9 on four different rootstocks, BaiMao No. 2 (BM2), BaiYeDanCong (BY), HeiYeShuiXian (HY), and WuLingHong (WLH)] were chosen as materials. In total, 32 differential metabolites were identified, including phenolic acids, flavan-3-ols, dimeric catechins, flavonol and flavonol/flavone glycosides, etc. Partial least squares discrimination analysis and hierarchical cluster analysis showed various effects of different rootstocks on metabolites. Thereinto, rootstocks of WLH and BY showed extremely outstanding performance in up- and downregulating these metabolites, respectively. Differential metabolites were enriched into three crucial pathways, including biosynthesis of phenylpropanoids, flavonoid biosynthesis, and flavone and flavonol biosynthesis, which might influence the quality of tea. This study provides a theoretical basis for grafting-related variations of non-volatile metabolites in fresh tea leaves.
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| http://dx.doi.org/10.1021/acs.jafc.9b01001 | DOI Listing |
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