Assessment of the Antimicrobial Potentiality and Functionality of Strains Isolated from the Conventional Inner Mongolian Fermented Cheese Against Foodborne Pathogens.

are amongst the diversified lactic acid bacteria (LAB) species which are being utilized abundantly in the food industry. Numerous strains have been reported to produce several antimicrobial compounds. Diacetyl, hydrogen peroxide, organic acids, as well as bacteriocins can also be exemplified by a variable spectrum of actions. The current study was intended to conduct the screening and characterization of antimicrobial prospective of from traditional Inner Mongolian fermented hard cheese. Foodborne pathogens, O157:H7, , and , were examined by using the Oxford cup technique and the mixed culture inhibition assays. The resulting analyses disclosed that KLDS1.0344 indicated broad antimicrobial spectrum against all selected pathogens as compared to other LAB used in this study. Additionally, the decrement of the pathogen population was observed up to 3.47 logs in mixed culture inhibition assays. KLDS 1.0344 acid production was recorded up to 71.8 ± 3.59 °D in mixed culture while antimicrobial particles released in cell free supernatants demonstrated bacteriocin-like characteristics showing substantial pH stability (2.0-6.0), proteolytic enzyme reduced the antibacterial activity (15.2 ± 0.6 mm-20.4 ± 0.8 mm), heat stability (20 min at 120 °C) against selected pathogens. Moreover, the spectrum range of antimicrobial peptides after the partial purification was decreased as compared to the crude bacteriocin-like compound. The SDS-PAGE analysis showed the molecular weight range of partially purified bacteriocin from 12 to 45 kDa. After analyzing the obtained data from the current experimentation showed that the capability of KLDS 1.0344 to oppose the pathogen growth in vitro relies on the occurrence of organic acids along with bacteriocin-like compounds proving KLDS 1.0344 as a potentially appropriate candidate as an alternative bio-control agent against foodborne pathogens.