Molecular Characterization of Species Isolated from Sheep and Goats in Riyadh, Saudi Arabia.

Sarcocystosis is induced by species of , which is an intracellular protozoan parasite in the phylum Apicomplexa. The diversity and importance of species in sheep and goats in Saudi Arabia are poorly understood. In this study, the tongue, esophagus, heart, diaphragm, and skeletal muscles were collected from 230 sheep and 84 goats, and the tissues were examined for the presence of species by macroscopic examination and light microscopy. Microscopic species cysts were found in both sheep and goats. Transmission electron microscopy (TEM) revealed in sheep and in goats. species were confirmed for the first time in Saudi Arabian sheep and goats by molecular testing. was most closely related to with the COX1 sequences sharing 91.7% identity. A phylogenetic analysis produced similar results and indicated that the isolates were within a group of species in which dogs were the final host. Finally, the species cysts from sheep and goats could be grouped together, indicating that they were strongly related.