AI Article Synopsis
- Wolbachia-infected Aedes aegypti mosquitoes show lower rates of arbovirus infection and viral load compared to wild-type mosquitoes, even when co-infected with multiple viruses.
- The study demonstrated that co-infection with Zika virus (ZIKV) and dengue viruses (DENV-1 and DENV-3) does not hinder Wolbachia's ability to reduce viral transmission.
- Findings indicate that using Wolbachia-infected mosquitoes could effectively manage mosquito-borne diseases, particularly in regions facing multiple concurrent virus outbreaks.
Article Abstract
Background: Wolbachia's ability to restrict arbovirus transmission makes it a promising tool to combat mosquito-transmitted diseases. Wolbachia-infected Aedes aegypti are currently being released in locations such as Brazil, which regularly experience concurrent outbreaks of different arboviruses. A. aegypti can become co-infected with, and transmit multiple arboviruses with one bite, which can complicate patient diagnosis and treatment.
Methodology/principle Findings: Using experimental oral infection of A. aegypti and then RT-qPCR, we examined ZIKV/DENV-1 and ZIKV/DENV-3 co-infection in Wolbachia-infected A. aegypti and observed that Wolbachia-infected mosquitoes experienced lower prevalence of infection and viral load than wildtype mosquitoes, even with an extra infecting virus. Critically, ZIKV/DENV co-infection had no significant impact on Wolbachia's ability to reduce viral transmission. Wolbachia infection also strongly altered expression levels of key immune genes Defensin C and Transferrin 1, in a virus-dependent manner.
Conclusions/significance: Our results suggest that pathogen interference in Wolbachia-infected A. aegypti is not adversely affected by ZIKV/DENV co-infection, which suggests that Wolbachia-infected A. aegypti will likely prove suitable for controlling mosquito-borne diseases in environments with complex patterns of arbovirus transmission.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6544317 | PMC |
| http://dx.doi.org/10.1371/journal.pntd.0007443 | DOI Listing |
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