AI Article Synopsis
- Developed a sensitive fluorescent method to detect long noncoding RNAs (lncRNAs) using a unique signal amplification technique involving duplex-specific nuclease.
- The method has a high sensitivity level, achieving a detection limit as low as 0.081 fM.
- Successfully identified the endogenous lncRNA HOTAIR in cancer cells, offering a novel way to explore lncRNA functions in human diseases.
Article Abstract
We develop a new fluorescent method for sensitive detection of long noncoding RNAs (lncRNAs) on the basis of duplex-specific nuclease-actuated cyclic enzymatic repairing-mediated signal amplification. This method exhibits high sensitivity with a detection limit of 0.081 fM, and it can accurately detect the endogenous lncRNA HOTAIR in cancer cells, providing a new approach to study the physiological function of lncRNAs in human diseases.
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| http://dx.doi.org/10.1039/c9cc02939g | DOI Listing |
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