Adeno-Associated Virus (AAV) Vectors: Rational Design Strategies for Capsid Engineering.

Curr Opin Biomed Eng

Department of Bioengineering, Rice University, 6500 Main St., MS-142, Houston, TX 77030, USA.

Published: September 2018

Adeno-associated virus (AAV) consists of a simple genome, infects mammalian cells, displays nonpathogenicity in humans, and spans an array of serotypes and variants bearing distinct tissue tropisms. These attributes lend AAV tremendous promise as a gene delivery vector, further substantiated by its extensive testing in human clinical trials. Rational design approaches to capsid engineering leverage current scientific knowledge of AAV to further modulate, enhance and optimize the performance of the vectors. Capsid modification strategies include amino acid point mutations, peptide domain insertions, and chemical biology approaches. Through such efforts, insights regarding AAV capsid sequence-structure-function relationships can be learned. Developments over the last 5 years in rational design-based capsid engineering approaches will be presented and discussed.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6516759PMC
http://dx.doi.org/10.1016/j.cobme.2018.09.004DOI Listing

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