The Hiraoka transmission electron microscopy (TEM) grid staining apparatus, which allowed multiple grids to be batch stained together on a plate, is no longer commercially available. Because the need for such a device still exists, we developed a modified Hiraoka TEM grid staining apparatus by combining a Leica AC20 grid plate holder with a modified 3D printed grid loading holder and trays lined with Parafilm. This apparatus was then used to test a post-section contrasting method that uses gadolinium triacetate tetrahydrate solution, a non-radioactive uranyl acetate substitute, which produces similar staining results to uranyl acetate when used with lead stain in mouse ocular tissue for TEM.
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| http://dx.doi.org/10.1080/01478885.2017.1361117 | DOI Listing |
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