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Comparison of six methods of DNA extraction for the diagnosis of bovine brucellosis by real-time PCR. | LitMetric

Comparison of six methods of DNA extraction for the diagnosis of bovine brucellosis by real-time PCR.

Arch Microbiol

Laboratório Nacional Agropecuário de Minas Gerais (Lanagro/MG), Avenida Rômulo Joviano, Caixa Postal 50, Centro Pedro Leopoldo, MG, Brazil.

Published: October 2019

Brucellosis is an infectious disease caused by bacteria of the genus Brucella, which affects domestic animals and is transmissible to humans. The objective of this study was to evaluate six methods of DNA extraction directly from bovine tissue to detect Brucella spp. The Cq values for all samples were above 30 and varied according to the extraction kit used, but four kits showed no statistical difference in sensitivity. This work demonstrates the importance of choosing the best extraction kit before validation of a molecular diagnostic technique.

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http://dx.doi.org/10.1007/s00203-019-01675-3DOI Listing

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