AI Article Synopsis
- A new 96-well microtiter method is introduced for testing inhibitors of Saprolegnia biofilms, which is a significant shift from previous methods focusing on spore germination or mycelial growth.
- The study specifically explores the effects of propionic acid (PPA) on the viability of Saprolegnia biofilms, finding that PPA significantly reduces hyphae viability, especially when combined with boric acid.
- The results indicate that while PPA is effective, the control treatment with bronopol produced a higher percentage of non-viable hyphae after 6 and 12 hours of exposure, consistent across various testing methods.
Article Abstract
A quantitative and reproducible 96-well microtiter method that is easily adaptable for the screening of Saprolegnia biofilm inhibitors is described. As opposed to other methods previously developed for the screening of Saprolegnia inhibitors on spore germination or mycelial growth, this technique is of particular significance as it investigates potential inhibitors against surface-attached mycelial mats of Saprolegnia spp. (biofilm). In this study, we have investigated the effects of propionic acid (PPA) on reducing the viability of induced Saprolegnia biofilms using colorimetric MTS assay based on the reduction of tetrazolium salts. Viability of Saprolegnia hyphae in treated biofilms was reduced significantly following treatment with different PPA concentrations. The effect was enhanced after combining each of the tested PPA concentrations with 500 mg/L of boric acid (BA). However, the percentage of non-viable hyphae was still higher in 200 mg L bronopol-treated biofilms (positive control) following 6- and 12-hr exposure. Similar results were observed using other recently described fluorescence-based assays for viability.
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