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Numb confers to inhibit epithelial mesenchymal transition via β-catenin/Lin28 signaling pathway in breast cancer. | LitMetric
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Numb confers to inhibit epithelial mesenchymal transition via β-catenin/Lin28 signaling pathway in breast cancer.

Xiao-Bo Hu Li-Zhi Ouyang Yue He Ming-Zhi Xia

Exp Mol Pathol

Breast Surgery Department I, Hunan Cancer Hospital, Changsha, Hunan 410013, PR China; Department of Mammary Gland of Hunan Tumor Hospital, Changsha 410013, PR China. Electronic address:

Published: August 2019

Objectives: This study aimed to investigate role of Numb in the epithelial mesenchymal transition (EMT) of breast cancer.

Methods: Numb and β-catenin were inhibited in MCF-7 cells using sh-RNA and overexpressed in T47D cells by pcDNA3.0-Numb, pcDNA3.0-β-catenin. Cell proliferation, invasion and migration were evaluated using MTT and Transwell assay, respectively. β-catenin, Lin28, and EMT related markers were determined using qRT-PCR and Western Blotting.

Results: Knockdown of Numb significantly promoted the proliferation, invasion and migration of MCF-7 cells, further increased the expression of β-catenin, Lin28, Snail-1, and N-cadherin, as well as decreased E-cadherin. In T47D cells transfected with pcDNA3.0-Numb, the results were quite the reverse.

Conclusions: Knockdown of Numb could promote the EMT of breast cancer cells via β-cateni/Lin28 signaling pathway.

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 Source
http://dx.doi.org/10.1016/j.yexmp.2019.104262DOI Listing

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