We describe the synthesis and application of 3-(trimethoxysilyl)propyl methacrylate (TPM) particles as a colloidal model system for three-dimensional (3D) confocal scanning laser microscopy. The effect of the initial TPM concentration on the growth and polydispersity of the particles and a recently developed solvent transfer method to disperse particles in a refractive index and density-matching solvent mixture are reviewed and discussed. To fully characterize the system as a colloidal model, we measure the pair potential between the TPM particles directly using optical tweezers. Finally, we use 3D confocal microscopy to image a sedimentation-diffusion equilibrium of TPM particles to characterize the phase behavior and particle dynamics through successful detection and tracking of all particles in the field of view.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.langmuir.9b00963 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Study on the structure and adsorption characteristics of the complex of modified Lentinus edodes stalks dietary fiber and tea polyphenol.
Food Chem
March 2025
School of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, PR China; Scientific Research Base of Edible Mushroom Processing Technology Integration of Ministry of Agriculture and Rural Affairs, Changchun 130118, China. Electronic address:
The waste Lentinus edodes stalks from Lentinus edodes processing were used as raw materials by the steam explosion to prepare modified Lentinus edodes stalks dietary fiber and combined with tea polyphenols to form the SE-DF-tea polyphenols complex (SE-DF-TPC). The SE-DF-tea polyphenols mixture (SE-DF-TPM) was prepared according to the complex's optimal adsorption conditions. Fluorescence microscopy, Fourier transform infrared spectroscopy, particle size measurement, thermogravimetric analysis, and X-ray diffraction were used to analyze its structure, and the thermal stability of the complex and its adsorption capacity for lipids, cholesterol, and cholates were studied.
View Article and Find Full Text PDFAssessment of airborne bacteria from a public health institution in Mexico City.
PLOS Glob Public Health
November 2024
Laboratorio de Microbiología Clínica, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Mexico City, México.
In this work, the composition of the bacterial community in the air of a hospital in Mexico City was evaluated using metabarcoding and proteomics approaches, along with the assessment of environmental factors such as temperature, humidity, and suspended particles. Two types of aerobiological samplers were used: Andersen One-Stage Viable Particle Sampler (AVPS) and Coriolis μ sampler (CμS-Sampler). Sampling was performed in four areas of the hospital: Floor 1 (F1), Floor 2 (F2), and Emergency Unit (EU), as well as outdoors (OH).
View Article and Find Full Text PDFSynergistic effects of bivalve and microalgae co-cultivation on carbon dynamics and water quality.
Mar Environ Res
October 2024
Tianjin Key Laboratory of Aqua-ecology and Aquaculture, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China; Key Laboratory of Smart Breeding (Co-construction By Ministry and Province, Ministry of Agriculture and Rural Affairs), Tianjin Agricultural University, Tianjin, 300384, China. Electronic address:
Aquaculture of bivalve shellfish and algae offers significant ecological benefits, yet the complex interactions between these organisms can substantially impact local carbon dynamics. This study investigated the effects of co-culturing four intertidal bivalve species Pacific oysters (Crassostrea gigas), Manila clams (Ruditapes philippinarum), Chinese clams (Cyclina sinensis), and hard clams (Mercenaria mercenaria) with microalgae (Isochrysis galbana) on specific water quality parameters, including total particulate matter (TPM), total organic matter (TOM), dissolved inorganic carbon (DIC), dissolved carbon dioxide (dCO), dissolved oxygen (DO), and ammonium (NH) concentrations. The bivalves were divided into smaller and larger groups and cultured under two conditions: with algae (WP) and without (NP), along with matched controls.
View Article and Find Full Text PDFQuantitation of DNA Binding Affinity Using Tethered Particle Motion.
Methods Mol Biol
July 2024
Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
The binding constant is an important characteristic of a DNA-binding protein. A large number of methods exist to measure the binding constant, but many of those methods have intrinsic flaws that influence the outcome of the characterization. Tethered particle motion (TPM) is a simple, cheap, and high-throughput single-molecule method that can be used to measure binding constants of proteins binding to DNA reliably, provided that they distort DNA.
View Article and Find Full Text PDFTethered Particle Motion Analysis of DNA-Binding Properties of Architectural Proteins.
Methods Mol Biol
July 2024
Leiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Architectural DNA-binding proteins are key to the organization and compaction of genomic DNA inside cells. Tethered particle motion (TPM) permits analysis of DNA conformation and detection of changes in conformation induced by such proteins at the single molecule level in vitro. As many individual protein-DNA complexes can be investigated in parallel, these experiments have high throughput.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!