Maintenance of the aseptic working field during endodontic treatment.

Acta Odontol Scand

Department of Endodontics, Institute of Clinical Dentistry, Faculty of Dentistry, University of Oslo, Oslo , Norway.

Published: October 2019

The aim of this clinical quality study was to determine whether the aseptic working field is maintained during the endodontic procedure. Bacterial samples were collected from the rubber dam of 27 patients during endodontic treatment performed by postgraduate students at the Department of Endodontics, University of Oslo. A bacterial sample was first obtained immediately after disinfection of the working field (A), and the second sample was collected just before obturation or dressing with calcium hydroxide cement (B). Aerobic cultivation technique and PCR were used for detection of bacterial growth and species. All samples were negative on culturing except in one case, which showed positive results with cultivation in both sample A and B. Specie detected with cultivation technique were . With PCR technique, 6 samples in 5 patients (11%), showed positive results. Species detected with PCR technique were and The present study showed that an aseptic working field was maintained throughout the endodontic procedure in 81% (22/27) of the cases after disinfection of the rubber dam.

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http://dx.doi.org/10.1080/00016357.2019.1606935DOI Listing

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