Development of -Specific Primers for Rapid Detection among Fungal Isolates in Citrus.

J Microbiol Biotechnol

Key Laboratory of Horticultural Plant Biology of the Ministry of Education, National Centre of Citrus Breeding, Huazhong Agricultural University, Wuhan 430070, P.R. China.

Published: June 2019

Blue mold in citrus is caused by . In this study, the -specific primers were developed for rapid detection based on the conserved genes and among genomes. The two primer pairs RPB1-a and RPB1-b proved to be specific to detect . The PCR assay among 39 fungal isolates and the colonial, pathogenic morphologies and molecular methods validated the specificity and reliability of these two primer pairs. This report provided a method and -specific primers, which might greatly contribute to citrus postharvest industry.

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http://dx.doi.org/10.4014/jmb.1904.04021DOI Listing

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