AI Article Synopsis
- Neural stem cell (NSC) transplantation shows promise for treating Parkinson's disease, but many NSCs differentiate into glial cells and die due to inflammation post-transplant.
- In this study, researchers transplanted NSCs and microglial cells that overexpress the Nurr1 gene into the brains of rats with Parkinson's, evaluating the effects through various scientific methods.
- Results indicated that this combined therapy improved behavior in PD rats, increased dopamine-producing cells, and reduced inflammatory cells, suggesting a new strategy for cell replacement therapy in Parkinson's disease.
Article Abstract
Background: Neural stem cells (NSCs) transplantation is considered a promising treatment for Parkinson's disease. But most NSCs are differentiated into glial cells rather than neurons, and only a few of them survive after transplantation due to the inflammatory environment.
Methods: In this study, neural stem cells (NSCs) and microglial cells both forced with the Nurr1 gene were transplanted into the striatum of the rat model of PD. The results were evaluated through reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunofluorescence analysis.
Results: The behavioral abnormalities of PD rats were improved by combined transplantation of NSCs and microglia, both forced with Nurr1. The number of tyrosine hydroxylase+ cells in the striatum of PD rats increased, and the number of Iba1+ cells decreased compared with the other groups. Moreover, the dopamine neurons differentiated from grafted NSCs could still be detected in the striatum of PD rats after 5 months.
Conclusions: The results suggested that transplantation of Nurr1-overexpressing NSCs and microglia could improve the inhospitable host brain environments, which will be a new potential strategy for the cell replacement therapy in PD.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6930818 | PMC |
| http://dx.doi.org/10.1111/cns.13149 | DOI Listing |
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