Production of butanol for fuel via the conventional Acetone-Butanol-Ethanol fermentation has been considered economically risky because of a potential oversupply of acetone. Alternatively, acetone is converted into isopropanol by specific solventogenic Clostridium species in the Isopropanol-Butanol-Ethanol (IBE) fermentation. This route, although less efficient, has been gaining attention because IBE mixtures are a potential fuel. The present work is dedicated to reviewing past and recent advances in microorganisms, feedstock, and fermentation equipment for IBE production. In our analysis we demonstrate the importance of novel engineered IBE-producing Clostridium strains and cell retention systems to decrease the staggering number of fermentation tanks required by IBE plants equipped with conventional technology. We also summarize the recent progress on recovery techniques integrated with fermentation, especially gas stripping. In addition, we assessed ongoing pilot-plant efforts that have been enabling IBE production from woody feedstock.
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http://dx.doi.org/10.1016/j.biortech.2019.121425 | DOI Listing |
Bioresour Technol
April 2023
Tidetron Bioworks Technology (Guangzhou) Co., Ltd., Guangzhou Qianxiang Bioworks Co., Ltd., Guangzhou, Guangdong 510000, China. Electronic address:
To elevate the efficiency of acetone-butanol-ethanol (ABE) fermentation by the wild-type strain WK, an optimal co-utilization system (20 mM Fe and 5 g/L butyrate) was established to bring about a 22.22% increment in the yield of ABE mixtures with a significantly enhanced productivity (0.32 g/L/h).
View Article and Find Full Text PDFFEMS Microbes
April 2022
CD-Laboratory for Biotechnology of Glycerol, BOKU-University of Natural Resources and Life Sciences, Vienna, Austria.
Converting plant biomass into biofuels and biochemicals microbial fermentation has received considerable attention in the quest for finding renewable energies and materials. Most approaches have so far relied on cultivating a single microbial strain, tailored for a specific purpose. However, this contrasts to how nature works, where microbial communities rather than single species perform all tasks.
View Article and Find Full Text PDFBioresour Technol
January 2022
Laboratory of Optimization, Design, and Advanced Control - Fermentation Division (LOPCA-Ferm), School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address:
The Isopropanol-Butanol-Ethanol productivity by solventogenic clostridia can increase when cells are immobilized on low-cost, renewable fibrous materials; however, butanol inhibition imposes the need for dilute sugar solutions (less than40 g/L). To alleviate this problem, the in-situ vacuum product recovery technique was applied to recover IBE in repeated-batch cultivation of Clostridium beijerinckii DSM 6423 immobilized on sugarcane bagasse. Five repeated batch cycles were conducted in a 7-L bioreactor containing P2 medium (∼60 g/L glucose) and bagasse packed in 3D-printed concentric annular baskets.
View Article and Find Full Text PDFGenomics
January 2021
Department of Biotechnology, University of Chemistry and Technology Prague, Technicka 5, 166 28 Prague, Czech Republic.
Clostridium diolis DSM 15410 is a type strain of solventogenic clostridium capable of conducting isopropanol-butanol-ethanol fermentation. By studying its growth on different carbohydrates, we verified its ability to utilize glycerol and produce 1,3-propanediol and discovered its ability to produced isopropanol. Complete genome sequencing showed that its genome is a single circular chromosome and belongs to the cluster I (sensu scricto) of the genus Clostridium.
View Article and Find Full Text PDFBioresour Technol
January 2021
Laboratory of Optimization, Design, and Advanced Control - Fermentation Division (LOPCA-Ferm), School of Chemical Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil. Electronic address:
Enzymatic hydrolysis of lignocellulose under industrial conditions is prone to contamination by lactic acid bacteria, and in this study, a cellulose hydrolysate produced from dilute-acid pretreatedsugarcane bagasse contained 13 g/L lactic acid and was used for IBE production by Clostridium beijerinckii DSM 6423. In fermentation of the cellulose hydrolysate supplemented with sugarcane molasses for nutrients and buffering of the medium (40 g/L total sugar), 92% of the lactic acid was consumed, and the butanol yield was as high as 0.28 (7.
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