Regulatory effects of CCDC3 on proliferation, migration, invasion and EMT of human cervical cancer cells.

Objective: To elucidate the potential effects of Coiled coil domain-containing 3 (CCDC3) on proliferative, migratory, invasive potentials and epithelial-mesenchymal transition (EMT) of human cervical cancer cells.

Materials And Methods: Protein and mRNA levels of CCDC3 in C33 and HeLa cells were determined by quantitative Real-time polymerase chain reaction (qRT-PCR) and Western blot, respectively. Proliferative capacity and clonality of C33 and HeLa cells transfected with sh-CCDC3 were evaluated by cell counting kit-8 (CCK-8) and colony formation assay, respectively. Transwell assay and wound healing assay were conducted to determine the invasive and migratory potentials of cervical cancer cells with CCDC3 knockdown. Protein expressions of EMT-related genes in C33 and HeLa cells with CCDC3 knockdown were determined by Western blot.

Results: Transfection of sh-CCDC3 in C33 and HeLa cells markedly inhibited CCDC3 expression compared with those transfected with sh-EGFP. CCDC3 knockdown remarkably attenuated proliferative, migratory and invasive capacities. Moreover, CCDC3 knockdown inhibited protein levels of EMT-related genes in C33 and HeLa cells.

Conclusions: Low expression of CCDC3 attenuated proliferative, migratory, invasive potentials and EMT of cervical cancer cells. Hence, CCDC3 may be utilized as a novel therapeutic target for cervical cancer.