High Resolution Melting (HRM) analysis is a post-PCR analysis method used for identifying genetic variation in nucleic acid sequences. These data are presenting the identity of the 33 samples used for a qPCR-HRM and a nested snapback methods validation. In addition we are presenting the high resolution melting profiles of (Poc) and (Pow) in the following conditions: after a direct qPCR run and after a nested snapback run. The qPCR-HRM of artificial mixture of Poc and Pow plasmids (200 copies/μl, each) at different proportions are showing the melting pattern of co-infections with both species. The sequencing methodology of the c gene fragment of 12 randomly selected samples is described and their likeness to published sequences is shown in a maximum likelihood tree. "Novel high resolution melting and snapback assays for simultaneous detection and differentiation of Plamodium ovale spp." [1].
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6502744 | PMC |
| http://dx.doi.org/10.1016/j.dib.2019.103937 | DOI Listing |
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