The current research investigated the possibility of valorizing cocoa pods residues through anaerobic digestion and the possibility of increasing the biogas yield by hydrothermal pretreatment. Using a central composite surface response methodology, the effect of temperature and reaction time on the hydrothermal pretreatment process was studied. Temperature and reaction time was varied between 150-220 °C and 5-15 min respectively. The result show that untreated cocoa pods residues has a biogas potential of 357 l(N)/kgVS and a methane content of 55%. The effect of hydrothermal pretreatment on the biogas yield was diverse. Severities below 3.0 resulted in increased biogas yield. However, higher severity resulted in lower biogas yield. The optimum biogas yield (526.38 l(N)/kgVS) was obtained at 150 °C and 15 min, which represents a severity of 2.65.
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http://dx.doi.org/10.1016/j.wasman.2019.03.034 | DOI Listing |
Folia Microbiol (Praha)
January 2025
Biofuels Institute, School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China.
Ginsenoside Rh2(S) is well-known for its therapeutic potential against diverse conditions, including some cancers, inflammation, and diabetes. The enzymatic activity of uridine diphosphate glycosyltransferase 51 (UGT51) from Saccharomyces cerevisiae plays a pivotal role in the glycosylation process between UDP-glucose (donor) and protopanaxadiol (acceptor), to form ginsenoside Rh2. However, the catalytic efficiency of the UGT51 has remained a challenging task.
View Article and Find Full Text PDFBiotechnol Biofuels Bioprod
January 2025
Environmental Biotechnologies, Institute of Microbiology (IM) DACD Campus Mendrisio, University of Applied Sciences and Arts of Southern Switzerland SUPSI, Via Flora Ruchat-Roncati, 6850, Mendrisio, Switzerland.
This study explores the use of conductive material in scaling up anaerobic digestion for enhanced biogas production. Focusing on Direct Interspecies Electron Transfer (DIET), the research employs a syntrophic DIET-able consortium formed by Shewanella oneidensis and Methanosarcina barkerii in 3.8-L experiments utilizing reticulated vitreous carbon (RVC) as conductive material.
View Article and Find Full Text PDFBiotechnol Biofuels Bioprod
January 2025
Aquaculture Department, Faculty of Fisheries, Ege University, 35040, Izmir, Türkiye.
For biofuels and nutraceuticals, the green microalga Haematococcus pluvialis (Chlorophyceae) is a prospective source of biomass and lipids. This study examined how biomass production and lipid accumulation were affected by temperature (10 °C, 20 °C, and 30 °C) and potassium nitrate (KNO₃) concentrations (0.41 g/L, 0.
View Article and Find Full Text PDFBiotechnol Biofuels Bioprod
January 2025
Manchester Institute of Biotechnology, The University of Manchester, 131 Princess Street, Manchester, M1 7DN, UK.
Background: The climate crisis and depleting fossil fuel reserves have led to a drive for 'green' alternatives to the way we manufacture chemicals, and the formation of a bioeconomy that reduces our reliance on petrochemical-based feedstocks. Advances in Synthetic biology have provided the opportunity to engineer micro-organisms to produce compounds from renewable feedstocks, which could play a role in replacing traditional, petrochemical based, manufacturing routes. However, there are few examples of bio-manufactured products achieving commercialisation.
View Article and Find Full Text PDFBiotechnol Biofuels Bioprod
January 2025
Department of Animal Science and Technology, University of Northwest A&F, Yangling, 712100, Shaanxi, China.
Antimicrobial peptides (AMPs) are renowned for their potent bacteriostatic activity and safety, rendering them invaluable in animal husbandry, food safety, and medicine. Despite their potential, the physiological toxicity of AMPs to host cells significantly hampers their biosynthetic production. This study presents a novel approach for the biosynthesis of the antimicrobial peptide Kiadin by engineering a DAMP4-DPS-Kiadin fusion protein to mitigate host cell toxicity and achieve high-level expression.
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