AI Article Synopsis

  • - The study investigates the allergenic properties and elemental composition of Ailanthus altissima pollen (AAP) over two years (2014 and 2015), highlighting AAP as a significant aeroallergen.
  • - A variety of techniques, including gel-based analysis and energy-dispersive X-ray (EDX) analysis, revealed notable differences in allergen levels and the presence of pollutants like nickel and lead between the two years.
  • - The findings emphasized the role of specific proteins associated with allergies and suggest that monitoring changes in such invasive plant species could enhance understanding and management of environmental allergens.

Article Abstract

The Ailanthus altissima pollen (AAP) has been reported as an emerging aeroallergen worldwide. This paper aims at examining the allergen pattern and the elemental composition of A. altissima pollen collected during two consecutive seasons (2014 and 2015). A gel-based allergomic study and SEM coupled to energy-dispersive X-ray (EDX) analysis have been carried out in order to evaluate the allergenic and elemental composition of AAP in two consecutive years. The IgE reactive patterns of 2014 and 2015 AAP PBS extracts were compared using the serum of a 31-year-old woman suffering from severe pollinosis symptoms to AAP. The EDX analysis revealed an important year-to-year variation in the ratios of some polluting elements such as nickel, sulfur, aluminum, lead, and copper. Gel alignments and comparative immunoproteomic analyses showed differential protein expression and IgE reactive patterns between AAPs collected in 2014 and 2015 pollinating seasons. From 20 distinct IgE-reactive spots detected in AAP extracts, 13 proteins showed higher expression in 2014 sample, while 7 allergen candidates exhibited an increased expression in AAP collected in 2015. Matrix-assisted laser desorption ionization-MS/MS analyses led to the identification of 13 IgE-binding proteins with confidence, all belonging to well-known allergenic protein families, i.e., enolase, calreticulin, and pectate lyase. Overall, the 2014 AAP showed higher concentrations of urban polluting elements as well as an increased expression of allergenic pectate lyase isoforms of about 52 kDa. This study demonstrates that the implementation of allergomic tools for the safety assessment of newly introduced and invasive plant species would help to the comprehensive monitoring of proteomic and transcriptomic alterations involving environmental allergens.

Download full-text PDF

Source
http://dx.doi.org/10.1007/s10661-019-7458-4DOI Listing

Publication Analysis

Top Keywords

altissima pollen
12
2014 2015
12
year-to-year variation
8
ailanthus altissima
8
allergomic study
8
elemental composition
8
edx analysis
8
ige reactive
8
reactive patterns
8
polluting elements
8

Similar Publications

Trees Are a Major Foraging Resource for Honeybees in the City.

Plants (Basel)

November 2024

Division of Structural and Functional Botany, Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030 Vienna, Austria.

Large cities are typically characterized by a mosaic of green spaces that hold a remarkable variety of native and "exotic" plants. Urban beekeeping has gained increasing popularity. In order to characterize the "urban" in the honey, pollen diversity in 50 honey samples from 18 apiary locations in Vienna, Austria, was microscopically analyzed.

View Article and Find Full Text PDF

Urban areas are often hotspots for the dissemination of non-native (invasive) plant species, some of which release (potentially) allergenic pollen. Given the high population density in cities, a considerable number of people can be regularly and potentially intensively exposed to the pollen from these plants. This study delves into the Tree-of-Heaven (Ailanthus altissima, [Mill.

View Article and Find Full Text PDF

Purpose: is one of the world's most invasive species with a globally problematic spread. Pollen is dispersed locally and partially airborne. We aimed at investigating if (i) pollen can be detected in relevant quantity in the air and if (ii) sensitization to can be detected in patients with seasonal exacerbation of atopic diseases.

View Article and Find Full Text PDF

Transcriptome sequencing and microsatellite marker discovery in Ailanthus altissima (Mill.) Swingle (Simaroubaceae).

Mol Biol Rep

March 2021

Fairy Lake Botanical Garden, Shenzhen & Chinese Academy of Sciences, 518004, Shenzhen, People's Republic of China.

Ailanthus altissima Swingle, is a tree species native to East Asia and has a great potential in decorative, bioenergy and industrial applications in many countries. To date, despite its commercial importance, the genomic and genetic resources available for this species are still insufficient. In this study, we characterized the transcriptome of A.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!