Objective: Use next-generation sequencing to develop microsatellite loci that will provide the variability necessary for studies of genetic diversity and population connectivity of two New World vulture species.
Results: We characterized 11 microsatellite loci for black vultures (Coragyps atratus) and 14 loci for turkey vultures (Cathartes aura). These microsatellite loci were grouped into 3 multiplex panels for each species. The number of alleles among black vulture samples ranged from 2 to 11, and 3 to 48 among turkey vulture samples.
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http://dx.doi.org/10.1186/s13104-019-4295-z | DOI Listing |
Sci Rep
December 2024
Department of Forensic Medicine, Guizhou Medical University, Guiyang, 550025, China.
Multi-insertion/deletion polymorphisms (Multi-InDels), as the novel genetic markers, show great potential in forensic research. Whereas, forensic researchers mainly focus on the multi-InDels on the autosomes, which can provide relatively limited information in some complex paternity cases. In this study, a novel X chromosomal multi-InDel multiplex amplification system was designed, containing 22 multi-InDels and one STR locus on the X chromosome.
View Article and Find Full Text PDFSheng Wu Gong Cheng Xue Bao
December 2024
College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, Guangdong, China.
To clarify the genetic diversity and structure of the nucleus population of F1-generation , this study utilized 15 pairs of highly polymorphic microsatellite primers to analyze the simple sequence repeat (SSR) markers and genetic diversity in 15 full-sib families of . . A total of 112 alleles () and 60.
View Article and Find Full Text PDFBiotechniques
December 2024
Department of Biology, Montclair State University, Montclair, NJ, USA.
Microsatellites are present in mitochondria, chloroplast, and nuclear DNA, but nuclear microsatellites are more useful genetic tools than those in plastids or mitochondria. Plastid and mitochondrial microsatellites have been identified in the model plant (liverwort), but no laboratory has published information on nuclear microsatellite loci. The aim of this study was to detect novel nuclear markers in the most commonly employed liverwort species, design PCR primers that would allow amplification, and characterize the subsequently generated loci.
View Article and Find Full Text PDFJ Mol Diagn
January 2025
Kunming Medical University, Kunming, China; Department of Colorectal Surgery, The Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming, China. Electronic address:
Microsatellite instability (MSI) detection using tumor tissue is a well-established prognostic and predictive biomarker for certain types of cancers. However, tumor tissue samples are less convenient to obtain than blood plasma samples. The main challenge facing next-generation sequencing-based MSI detection in blood plasma samples is the ultralow signal/noise ratio in plasma cell-free DNA (cfDNA).
View Article and Find Full Text PDFEffective conservation strategies inherently depend on preserving populations, which in turn requires accurate tools for their detection. Beluga whales () inhabit the circumpolar Arctic and form discrete summer aggregations. Previous genetic studies using mitochondrial and microsatellite loci have delineated distinct populations associated to summer aggregations but the extent of dispersal and interbreeding among these populations remains largely unknown.
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