How can engineers help invent tools for neuroscience? We here explore a model for how engineers can choose problems and map out possible technologies that help address them. We also discuss design principles of tools and the role of failure.
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| http://dx.doi.org/10.1016/j.neuron.2019.03.023 | DOI Listing |
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Precise Identification of Native Peptides with Posttranslational Proline Hydroxylation by Nanopore.
Angew Chem Int Ed Engl
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Nanjing University, School of Chemistry and Chemical Engineering, No. 163 Xianlin Road, 210023, Nanjing, CHINA.
Hydroxylation, an extensive post-translational modification on proline, is critical for the modulation of protein structures, further dominating their functions in life systems. However, current mass spectrometry-based identification, could hardly distinguish hydroxylation from neighboring oxidation due to the same mass shifts, as well as challenges posed by low abundance and exogenous oxidation during sample preparation. To address these, an engineered nanopore was designed, capable of discriminating single hydroxyl group, to achieve the identification of proline hydroxylation on individual native peptides directly in the mixture.
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Graduate School of Interdisciplinary Science and Engineering in Health Systems, Okayama University, Okayama, Japan.
The production of disulfide-containing recombinant proteins often requires refolding of inclusion bodies before purification. A pre-refolding purification step is crucial for effective refolding because impurities in the inclusion bodies interfere with refolding and subsequent purification. This study presents a new pre-refolding procedure using a reversible S-cationization technique for protein solubilization and purification by reversed-phase high performance liquid chromatography.
View Article and Find Full Text PDFCaML: Chemistry-informed machine learning explains mutual changes between protein conformations and calcium ions in calcium-binding proteins using structural and topological features.
Protein Sci
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Department of Physics, University of Washington, Seattle, Washington, USA.
Proteins' flexibility is a feature in communicating changes in cell signaling instigated by binding with secondary messengers, such as calcium ions, associated with the coordination of muscle contraction, neurotransmitter release, and gene expression. When binding with the disordered parts of a protein, calcium ions must balance their charge states with the shape of calcium-binding proteins and their versatile pool of partners depending on the circumstances they transmit. Accurately determining the ionic charges of those ions is essential for understanding their role in such processes.
View Article and Find Full Text PDFEngineered protein G variants for multifunctional antibody-based assemblies.
Protein Sci
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Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois, USA.
We have developed a portfolio of antibody-based modules that can be prefabricated as standalone units and snapped together in plug-and-play fashion to create uniquely powerful multifunctional assemblies. The basic building blocks are derived from multiple pairs of native and modified Fab scaffolds and protein G (PG) variants engineered by phage display to introduce high pair-wise specificity. The variety of possible Fab-PG pairings provides a highly orthogonal system that can be exploited to perform challenging cell biology operations in a straightforward manner.
View Article and Find Full Text PDFSpatial and temporal analyses of Dermacentor variabilis (Say) (Acari: Ixodidae) in central and eastern Canada.
Med Vet Entomol
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Department of Population Medicine, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Dermacentor variabilis (Say) (Acari: Ixodidae) is a vector for pathogens that can impact human and animal health. The geographic range of this species is expanding, but there are some areas with limited up-to-date information on the distribution of D. variabilis.
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