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Bench-to-bedside optimization of mesenchymal stem cell isolation, processing, and expansion for administration. | LitMetric
In this study, we aimed at identifying the optimal conditions for isolation, processing and expansion of mesenchymal stem cells (MSCs). Porcine bone marrow was obtained from either small- or large-volume bone marrow aspirate (BMA). Next, three BMA processing methods were compared. Finally, the best condition was selected from various culture parameters, including basal media, supplementation and seeding density. Our results demonstrate that a small-volume BMA and direct plating yields significantly higher concentration of MSCs. Basal media supplementation with 10% platelet lysate and seeding density of 1000 cells/cm can generate large numbers of multipotent MSCs with augmented function and low population doublings. This work provides guidance for preparation of robust MSCs for future clinical trials.
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