A new type of dimeric cyclic naphthalene diimide derivatives (cNDI-dimers) carrying varied linker length were designed and synthesized to recognize dimeric G-quadruplex structures. All of the cNDI-dimers exhibited a high preference for recognizing G-quadruplex structures, and significantly enhanced the thermal stability of the dimeric G-quadruplex structure over the cNDI monomer by increasing the melting temperature by more than 23 °C, which indicated the strengthened ability of cNDI dimers for stabilizing dimeric G-quadruplex. cNDI dimers also showed a stronger ability to inhibit telomerase activity and stop telomere DNA elongation than cNDI monomer, which showed an improved anticancer potentiality for further therapeutic application.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/chem.201901468 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
When annealing is detrimental: The case of HMGB1-targeting G-quadruplex aptamers.
Int J Biol Macromol
December 2024
Department of Chemical Sciences, University of Napoli Federico II, 80126 Napoli, Italy. Electronic address:
In this work, we present the case of the G-quadruplex(G4)-forming aptamers we recently identified for the recognition of HMGB1, protein involved in inflammation, autoimmune diseases and cancer. These aptamers were previously analyzed, without annealing them, after proper dilution of the stock solution in a pseudo-physiological buffer mimicking the extracellular environment where the protein exerts its pathological activity, and showed high thermal stability and nuclease resistance, good protein affinity and remarkable in vitro activity. These features were more marked for the aptamers forming dimeric, parallel G4 structures in solution.
View Article and Find Full Text PDFRNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed?
Nucleic Acids Res
November 2024
Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 612 65 Brno, Czech Republic.
Article Synopsis
- - G-quadruplexes (G4s) in RNA are becoming important targets for cancer and disease therapies, with GG motifs being more common than GGG motifs in RNA sequences.
- - Recent findings indicate that DNA cannot make stable two-tetrad G4 structures, raising questions about the stability of RNA GG motifs and their potential regulatory functions.
- - This study reveals that RNA GG motifs typically don't form stable intramolecular G4s by themselves; instead, they need to dimerize to create stable structures, which may play a role in RNA aggregation during cellular stress.
Cu-mediated telomeric dimeric G-quadruplex DNAzyme for highly sensitive colorimetric detection of deferasirox.
Talanta
February 2025
Pharmaceutical College, Guangxi Key Laboratory of Bioactive Molecules Research and Evaluation, Key Laboratory of Micro-Nanoscale Bioanalysis and Drug Screening of Guangxi Higher Education, Guangxi Medical University, Nanning, 530021, China.
Deferasirox (DEF) is an important iron chelator for treatment of iron overload-related diseases. Monitoring DEF concentration in human serum will provide some valuable information for clinical diagnosis and therapy of such diseases. In this study, we developed a peroxidase-mimicking colorimetric sensor for the detection of DEF by simple assembly of a telomeric dimeric G-quadruplex DNAzyme with Cu.
View Article and Find Full Text PDFFluorescence detection of adenosine triphosphate based on dimeric G-quadruplex.
Spectrochim Acta A Mol Biomol Spectrosc
February 2025
School of Life Sciences, Central South University, Changsha, China. Electronic address:
Article Synopsis
- ATP is a crucial energy carrier in living organisms, linked to various biological processes and diseases, making it an important biomarker for cell viability and food freshness.
- * The study presents a new ATP detection sensor that utilizes the fluorescence change of dimeric G-quadruplex (Di-G4) in the presence of ATP, showing a linear relationship between fluorescence and ATP concentrations.
- * When tested on apples, this novel method demonstrated reliable results when compared to traditional ATP assay kits, indicating its potential for practical applications.
Selective Recognition of the Dimeric NG16 Parallel G-Quadruplex Structure Using Synthetic Turn-On Red Fluorescent Protein Chromophore.
Biochemistry
November 2024
Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India.
Red fluorescent protein (RFP)-based fluorescent probes that can selectively interact with specific nucleic acids are of great importance for therapeutic and bioimaging applications. Herein, we have reported the synthesis of RFP chromophores for selective recognition of G-quadruplex nucleic acids in vitro and ex vivo. We identified as a fluorescent turn-on probe that binds to the dimeric NG16 parallel quadruplex with superior selectivity and sensitivity over various parallel, antiparallel, and hybrid topologies.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!