1.8 μm Silica hydride particles have been derivatized with vancomycin and applied to the enantioseparation of some racemic herbicides and nonsteroidal anti-inflammatory drugs (NSAIDs) by nano-liquid chromatography. The chiral stationary phase (CSP) was packed for only 11 cm and the enantiomers were separated utilizing a laboratory-assembled instrumentation. The new CSP was very effective for the separation of the above mentioned acidic compounds, while poor resolutions were obtained for basic compounds. Mixtures of acetate buffer with methanol or acetonitrile allowed the chiral resolution of all compounds. Fast chiral separation of a NSAIDs-related compound can be achieved in less than 60 s.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-4939-9438-0_13 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Utility of "flip-flop" chromatography employing silica hydride stationary phases with simultaneous photodiode array ultraviolet and single quadrupole mass detection for the analysis of seized drugs.
J Chromatogr A
September 2023
Department of Forensic Sciences, The George Washington University, Washington, DC 20007, USA. Electronic address:
For confirmation and/or screening purposes, rapid, selective, and precise chromatographic methods are required. In this vein, the utility of SiH columns (C18, UDC Cholesterol, and Diamond Hydride) with photodiode array UV absorption and single quadrupole MS detection for multi-modal separation of representative drugs from different drug classes on a single column using the same solvent reservoirs was investigated. For a conventional two column approach employing a combination of conventional C18 and silica columns operating in both the reversed phase chromatographic and hydrophilic interaction chromatographic modes, gradient analysis is required for the first column and there is a lack of retention on the second column for non-amine analytes.
View Article and Find Full Text PDFApplication of a Cholesterol-Based Stationary Phase for the Analysis of Brevetoxins.
J Sep Sci
January 2023
University of North Carolina Wilmington, College of Health and Human Services, Wilmington, North Carolina, USA.
A high-performance liquid chromatography protocol for the analysis of brevetoxins has been developed using a silica hydride-based cholesterol column. Brevetoxins are neurotoxins produced by harmful algae that have additional potential as drugs for a number of illnesses/diseases. To develop the optimum conditions, a number of different experimental approaches were tested.
View Article and Find Full Text PDFSilica Hydride: A Separation Material Every Analyst Should Know About.
Molecules
December 2021
Department of Chemistry, San Jose State University, San Jose, CA 95192, USA.
This review describes the development, special features and applications of silica hydride-based stationary phases for HPLC. The unique surface of this material is in contrast to ordinary, standard silica, which is the material most frequently used in modern HPLC stationary phases. The standard silica surface contains mainly silanol (Si-OH) groups, while the silica hydride surface is instead composed of silicon-hydrogen groups, which is much more stable, less reactive and delivers different chromatographic and chemical characteristics.
View Article and Find Full Text PDFDispersive liquid-liquid microextraction, an effective tool for the determination of synthetic cannabinoids in oral fluid by liquid chromatography-tandem mass spectrometry.
J Pharm Anal
June 2021
Teaching Committee of Ph.D. School in Natural Science and Engineering University of Verona, Verona, Italy.
In the present work, dispersive liquid-liquid microextraction (DLLME) was used to extract six synthetic cannabinoids (JWH-018, JWH-019, JWH-073, JWH-200, or WIN 55,225, JWH-250, and AM-694) from oral fluids. A rapid baseline separation of the analytes was achieved on a bidentate octadecyl silica hydride phase (Cogent Bidentate C; 4.6 mm × 250 mm, 4 μm) maintained at 37 °C, by eluting in isocratic conditions (water:acetonitrile (25:75, )).
View Article and Find Full Text PDFChromatographic characterization of a silica hydride-based amide stationary phase.
J Sep Sci
July 2021
MicroSolv Technology Corporation, Leland, NC, USA.
An amide phase based on a porous silica hydride support material is tested for retention characteristics in both the reversed-phase and aqueous normal-phase modes. A series of retention maps (capacity factor vs. mobile phase composition) were obtained using reference standards of varying analyte sizes, functionalities, and polarities.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!