Studies on the antifungal and serotonin receptor agonist activities of the secondary metabolites from piezotolerant deep-sea fungus sp.

The potent antifungal agent sesquiterpenes and serotonin 5-HT agonist ascotricin were produced by a newly isolated deep-sea fungus sp. This fungus was isolated from deep-sea sediment collected at a depth of 1235 m and characterized. Piezotolerance was successfully tested under high pressure-low temperature (100 bar pressure and 20ºC) microbial cultivation system. Production of secondary metabolites was enhanced at optimized culture conditions. The antifungal activity of sesquiterpenes was studied using the model system. The sesquiterpenes affected the virulence of and prolonged the life of the host . These findings suggest that sesquiterpenes are attractive antifungal drug candidates. The 5-HT receptor agonist is a potential target for the development of drugs for a range of central nervous system disorders. The interaction of 5-HT agonist ascotricin with the receptor was studied through bioinformatic analysis. The molecular docking and molecular dynamic simulation studies demonstrated that they fit into the serotonin 5-HT active site and the crucial amino acid residues involved in the interactions were identified. To our knowledge, this is first report of antifungal analysis of sesquiterpenes and studies of serotonin 5-HT receptor-ascotricin complex.