AI Article Synopsis
- A new promoter probe vehicle has been developed for highly sensitive measurement of transcriptional activity from random DNA fragments, allowing real-time analysis.
- This noninvasive and nondestructive system is especially useful for studying complex transcriptional regulation during the sporulation cycle in Bacillus species.
- Researchers isolated DNA fragments from B. megaterium and B. subtilis that can initiate transcription in both their parent organisms and E. coli, revealing key developmentally controlled promoter regions.
Article Abstract
We report the construction and use of a new promoter probe vehicle capable of allowing extremely sensitive measurements of transcriptional activity promoted from random, chromosomal DNA fragment inserts. Coupled with the advantage of sensitivity, the detection system is noninvasive, nondestructive, and provides real-time reportage of expression potential. These latter aspects make it an especially valuable system for a continuing analysis of the complex transcriptional regulation patterns now recognized as a dominant control feature during the differentiation and morphogenesis characteristic of the sporulation cycle in Bacillus species. In this respect we describe the isolation of DNA fragments from B. megaterium and B. subtilis capable of initiating transcription in both the respective parent organisms and, in certain instances, also in Escherichia coli. Detailed luminescence studies showed that several promoter regions which are entirely or substantially developmentally controlled were isolated.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC212120 | PMC |
| http://dx.doi.org/10.1128/jb.169.5.2165-2170.1987 | DOI Listing |
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