AI Article Synopsis
- Water hyacinth (Eichhornia crassipes) is being explored as a potential feedstock for ethanol production through a multi-step process involving acid hydrolysis and enzymatic saccharification.
- Optimal conditions for hydrolysis were found to be 8% slurry content, 200 mM HSO, at 160 °C for 20 minutes, yielding 48.2 g/L of monosaccharides.
- Fermentation using various yeast strains resulted in ethanol production, with adaptive evolution improving yields, indicating water hyacinth's viability as a sustainable biofuel source.
Article Abstract
Water hyacinth (Eichhornia crassipes) was used as a feedstock for ethanol production. The optimal hyper-thermal (HT) acid hydrolysis conditions were 8% (w/v) slurry content, 200 mM HSO, at 160 °C for 20 min and enzymatic saccharification for 48 h using an enzyme mixture of 20 units/mL Viscozyme L and Cellic C Tec2. After pretreatment, 48.2 g/L monosaccharides were obtained. Fermentation was conducted with wild and adapted Saccharomyces cerevisiae, Pichia stipitis and Candida lusitaniae. Wild-type S. cerevisiae, P. stipitis, and C. lusitaniae produced 15.3, 19.5 and 22.7 g/L of ethanol, respectively. Adaptive evolution was carried out on 6% (w/v) xylose. S. cerevisiae, P. sipitis and C. lusitaniae adapted to xylose produced 15.3, 21.4 and 23.9 g/L of ethanol with Y of 0.32, 0.44 and 0.49, respectively. These results indicate that water hyacinth has potential as a feed stock for ethanol.
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| http://dx.doi.org/10.1007/s00449-019-02136-3 | DOI Listing |
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