Collagen was extracted from bigeye tuna () skins by salting-out (PSC-SO) and isoelectric precipitation (PSC-IP) methods. The yield of the PSC-IP product was approximately 17.17% (dry weight), which was greater than the yield obtained from PSC-SO (14.14% dry weight). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis indicated that collagen from bigeye tuna skin belongs to collagen type I. Inductively coupled plasma mass spectrometry results indicate that the heavy metal abundance in PSC-IP was lower than the maximum acceptable amounts according to Chinese regulatory standards. In addition, results from a methylthiazolyldiphenyl-tetrazolium bromide assay and an in vitro scratch assay demonstrated that PSC-IP could promote the proliferation and migration of NIH-3T3 fibroblasts. Overall, results suggest PSC-IP could be used to rapidly extract collagen from marine by-products instead of traditional salting-out methods. Collagen from bigeye tuna skin may also have strong potential for cosmetic and biomedical applications.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6562556 | PMC |
| http://dx.doi.org/10.3390/md17050261 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Correlation between quality change and hydrogen sulfide in aquatic product: Detection of hydrogen sulfide and its potential applications using bigeye tuna (Thunnus obesus) model during cold storage.
Food Chem
December 2024
College of Food Science & Technology, Shanghai Ocean University, Shanghai 201306, China; The International Peace Maternity and Child Health Hospital, School of Medicine. Shanghai Jiao Tong University, Shanghai 200030, China. Electronic address:
Hydrogen sulfide (HS) is an metabolic product of tuna during the spoilage, and relationship between HS and tuna quality has not been specifically studied. This study detected changes in HS content, HS precursor substances, and related enzymes based on the formation pathway of HS. HS content increased of tuna resulted in significant increases in contents of cystathionine β-synthase, cystathionine γ-lyase, 3-mercapto pyruvate sulfotransferase, cysteine aminotransferase and methionine, while content of cysteine decreased which provided HS formation.
View Article and Find Full Text PDFEvidence of spillover benefits from large-scale marine protected areas to purse seine fisheries.
Science
December 2024
Oceans Department, Doerr School of Sustainability, Stanford University, Stanford, CA, USA.
Global tuna fisheries are valued at more than $40 billion, with the majority of this value derived from purse seine fisheries. Recently created large-scale marine protected areas are potentially big enough to protect highly migratory species such as tuna, possibly leading to increases in abundance (a conservation benefit) and consequent spillover near protected area boundaries (an economic benefit). Using publicly available data from nine large-scale marine protected areas across the Pacific and Indian oceans, we find that catch-per-unit-effort in tuna purse seine fisheries has increased by an average of 12 to 18% near protected area boundaries, and this increase declines with distance from the boundaries.
View Article and Find Full Text PDFDevelopment of Single-Nucleotide Polymorphism (SNP)-Based Species-Specific Real-Time PCR Assays for Authenticating Five Highly Priced Tuna.
Foods
November 2024
Key Laboratory of Testing and Evaluation for Aquatic Product Safety and Quality, Ministry of Agriculture and Rural Affairs, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Tuna are economically important as food resources in food markets. However, because tuna is often processed into steaks or fillets, the meat can be difficult to identify through morphological features. For effective fishery management and to protect the rights of consumers, it is necessary to develop a molecular method to accurately identify the species used in tuna products.
View Article and Find Full Text PDFDirect assessment of tropical tuna abundance from their associative behaviour around floating objects.
Proc Biol Sci
August 2024
MARBEC, Univ. Montpellier, CNRS, Ifremer, IRD , Sète, France.
Managing populations of wild harvested species requires the ability to regularly provide accurate abundance assessments. For most marine species, changes in abundance can only be monitored indirectly, using methods reliant on harvest-based indices, with significant inherent limitations surrounding the estimation and standardization of harvest effort. Tropical tunas are some of the most exploited marine species in the world and are among several species in critical need of alternative methods for estimating abundance.
View Article and Find Full Text PDFDifferentiation of three commercial tuna species through GC-Q-TOF and UPLC-Q/Orbitrap mass spectrometry-based metabolomics and chemometrics.
Food Chem
September 2024
College of Food Science and Engineering, Yazhou Bay Innovation Institute, Hainan Tropical Ocean University, Marine Food Engineering Technology Research Center of Hainan Province, Collaborative Innovation Center of Marine Food Deep Processing, Hainan Key Laboratory of Herpetological Research, Sanya 572022, China. Electronic address:
Food fraud is common in the tuna industry because of the economic benefits involved. Ensuring the authenticity of tuna species is crucial for protecting both consumers and tuna stocks. In this study, GC-Q-TOF and UPLC-Q/Orbitrap mass spectrometry-based metabolomics were used to investigate the metabolite profiles of three commercial tuna species (skipjack tuna, bigeye tuna and yellowfin tuna).
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!