AI Article Synopsis
- A sandwich ELISA was created to detect 1-30% bovine milk in ovine milk using polyclonal antibodies developed from rabbit serum against bovine whey proteins.
- These antibodies were purified and used to capture bovine proteins in a microtiter plate, where they facilitated further recognition through biotin-conjugated antibodies.
- Detection involved a biotin-peroxidase reaction that produced measurable optical density differences based on the amount of bovine milk present in the samples.
Article Abstract
A sandwich ELISA (enzyme-linked immunosorbent assay) was developed for the detection of defined amounts of bovine milk (1-30%) in ovine milk. Polyclonal antibodies were raised in rabbits against bovine whey proteins (BWP). Resultant antibodies were affinity purified by immunoadsorption of the crude antiserum onto columns containing immobilized ovine, caprine, and BWP, followed by elution of the bovine milk specific antibodies (anti-BWP) from the column containing the bovine proteins. The specific anti-BWP antibodies bound to the wells of a microtiter plate were used to capture the BWP from milk mixtures. Further immunorecognition of the captured proteins was attained with the same specific antibodies conjugated to biotin. ExtrAvidin-peroxidase was used to detect the biotinylated antibodies bound to their specific antigens. Subsequent enzymic conversion of substrate gave clear optical density differences when assaying mixtures containing variable amounts of bovine milk.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.4315/0362-028X-54.5.366 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!