In order to perform an exact surface profile measurement with a white-light scanning interferometer (WLSI), an actual optical path difference (OPD) changing with time is detected with an additional interferometer in which the light source of the WLSI and an optical band-pass filter are used. This interferometer is simply equipped in the WLSI and does not negatively influence the WLSI. The real OPD is easily calculated from an interference signal with the same signal processing as that in the WLSI. The interference signal of the WLSI is corrected with the real OPD values or the real scanning position values. The corrected interference signal with a constant sampling interval is obtained with an interpolation method. With this correction method, a surface profile with a step shape of 3-μm height is measured accurately with an error less than 2 nm.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/AO.58.003548 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A complementary experimental and computational study on methanol adsorption isotherms of H-ZSM-5.
Phys Chem Chem Phys
January 2025
UK Catalysis Hub, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, OX11 0FA, UK.
Methanol adsorption isotherms of fresh f-ZSM-5 and steamed s-ZSM-5 (Si/Al ≈ 40) are investigated experimentally at room temperature under equilibrium and by grand canonical Monte Carlo (GCMC) simulations with the aim of understanding the adsorption capacity, geometry and sites as a function of steam treatment (at 573 K for 24 h). Methanol adsorption energies calculated by GCMC are complemented by density functional theory (DFT) employing both periodic and quantum mechanics/molecular mechanics (QM/MM) techniques. Physical and textural properties of f-ZSM-5 and s-ZSM-5 are characterised by diffuse reflectance infrared Fourier transformed spectroscopy (DRIFTS) and N-physisorption, which form a basis to construct models for f-ZSM-5 and s-ZSM-5 to simulate methanol adsorption isotherms by GCMC.
View Article and Find Full Text PDFIntegrin α8 is a useful cell surface marker of alveolar lipofibroblasts.
Respir Res
January 2025
Department of Regenerative and Infectious Pathology, Hamamatsu University School of Medicine, 1-20-1 Handayama Chuo-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
Background: Recent advances in comprehensive gene analysis revealed the heterogeneity of mouse lung fibroblasts. However, direct comparisons between these subpopulations are limited due to challenges in isolating target subpopulations without gene-specific reporter mouse lines. In addition, the properties of lung lipofibroblasts remain unclear, particularly regarding the appropriate cell surface marker and the niche capacity for alveolar epithelial cell type 2 (AT2), an alveolar tissue stem cell.
View Article and Find Full Text PDFTwo-Level Spatially Localized Proximity Labeling for Cross-Biological-Hierarchy Measurement and Manipulation.
Angew Chem Int Ed Engl
January 2025
Nanjing University, School of Chemistry and Chemical Engineering, CHINA.
Proximity labeling (PL) has emerged as a powerful technique for the in situ elucidation of biomolecular interaction networks. However, PL methods generally rely on single-biological-hierarchy control of spatial localization at the labeling site, which limits their application in multi-tiered biological systems. Here, we introduced another enzymatic reaction upstream of an enzyme-based PL reaction and targeted the two enzymes to markers indicating different biological hierarchies, establishing a two-level spatially localized proximity labeling (P2L) platform for in situ molecular measurement and manipulation.
View Article and Find Full Text PDFHeparan Sulfate Proteoglycan Affinity of Adeno-Associated Virus Vectors: Implications for Retinal Gene Delivery.
Eur J Pharm Sci
January 2025
Department of Ophthalmology, LMU University Hospital, LMU Munich, Munich, Germany. Electronic address:
Adeno-associated virus (AAV)-based vectors have emerged as an effective and widely used technology for somatic gene therapy approaches, including those targeting the retina. A major advantage of the AAV technology is the availability of a large number of serotypes that have either been isolated from nature or produced in the laboratory. These serotypes have different properties in terms of sensitivity to neutralizing antibodies, cellular transduction profile and efficiency.
View Article and Find Full Text PDFEvaluation of a novel malaria anti-sporozoite vaccine candidate, R21 in Matrix-M adjuvant, in the UK and Burkina Faso: two phase 1, first-in-human trials.
Lancet Microbe
December 2024
Jenner Institute, University of Oxford-NIHR Oxford Biomedical Research Centre, Oxford, UK. Electronic address:
Background: Malaria remains a substantial public health burden among young children in sub-Saharan Africa and a highly efficacious vaccine eliciting a durable immune response would be a useful tool for controlling malaria. R21 is a malaria vaccine comprising nanoparticles, formed from a circumsporozoite protein and hepatitis B surface antigen (HBsAg) fusion protein, without any unfused HBsAg, and is administered with the saponin-based Matrix-M adjuvant. This study aimed to assess the safety and immunogenicity of the malaria vaccine candidate, R21, administered with or without adjuvant Matrix-M in adults naïve to malaria infection and in healthy adults from malaria endemic areas.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!