Airborne fungal spores are extensively reported as the elicitors of respiratory allergies in human. is one such fungal species reported for eliciting significant skin prick results from India. The present study aims to analyze the allergenic potential of followed by the identification of allergens. The total protein of was subjected to 1dimensional (1D) and 2D gel electrophoresis followed by corresponding IgE-specific immunoblots. We found 8 immunoreactive bands/zones in (1D) immunoblot using 11 -sensitised patient sera. In 1D immunoblot, a 34 kDa band was detected in >80% of the patients and hence considered as a potential allergen of . Corresponding 34 kDa spot in 2D-immunoblot was analyzed by mass spectrometric analysis and identified as Glyceraldehyde 3-phosphate dehydrogenase. The identified allergen holds the potential to instigate vaccine development for immunotherapy of sensitized patients.
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| http://dx.doi.org/10.1080/09603123.2019.1609658 | DOI Listing |
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