The intracellular K level in bacteria is strictly controlled by K uptake and efflux systems. Among these, KdpFABC is a high-affinity K transporter system that is generally activated by the KdpDE two-component system in response to K limitation stress. However, the regulatory mechanism remains obscure in bacteria lacking the genes. Here we report that the transcription of a operon is distinctively regulated by a cyclic diadenylate monophosphate (c-di-AMP) riboswitch located at the 5'-untranslated region of transcript, and binding of c-di-AMP to the riboswitch promotes its intrinsic termination that blocks the transcription. Further, the intracellular c-di-AMP concentration was found to decrease under the K limitation stress, leading to transcriptional read-through over the terminator to allow expression. This regulatory element is found predominantly in the group and correlate well with the K and c-di-AMP homeostasis that affects a variety of crucial cellular functions.
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| http://dx.doi.org/10.1038/s42003-019-0414-6 | DOI Listing |
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