Encapsulation of Silver Nanoparticles in Polylactic Acid or Poly(lactic-co-glycolic acid) and Their Antimicrobial and Cytotoxic Activities.

Encapsulation with biodegradable polymers is an alternative to reduce adverse effects and improve the physicochemical properties of metallic nanoparticles. In this study, spherical polymeric nanoparticles with an average size of 200 nm loaded with silver nanoparticles (AgNPs) were obtained. The antimicrobial activity against O157:H7, methicillin-resistant (MRSA), and yeasts as and was determined. MIC values of nanocomposites were between 0.01 to 1 g/mL, potentialized effect up to 500 times compared to free AgNPs. In addition, cytotoxic effect on 50% of murine fibroblast (CC) was obtained at a mean concentration of 9.57 g/mL of AgNPs (up to ~1000 times higher than MIC). Consequently, the polymeric nanocomposites loaded with AgNPs are a potential alternative in the development of new biocide agents on Candida species and pathogenic bacteria at non-toxic concentrations for mammalian cells such as murine fibroblasts.