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Disruption of the pleiotropic gene scoC causes transcriptomic and phenotypical changes in Bacillus pumilus BA06. | LitMetric

Disruption of the pleiotropic gene scoC causes transcriptomic and phenotypical changes in Bacillus pumilus BA06.

BMC Genomics

Key Laboratory for Bio-resources and Eco-Environment of the Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Sciences, Sichuan University, Chengdu, People's Republic of China.

Published: April 2019

AI Article Synopsis

  • - The study investigates the role of the scoC gene in the bacterium Bacillus pumilus, discovering that disrupting this gene increases extracellular protease activity but decreases cell motility, likely by affecting flagella formation.
  • - RNA sequencing revealed that more than 1,000 genes were affected at different growth phases, with the most significant changes occurring during the transition from exponential to stationary growth, particularly impacting protease gene regulation.
  • - The findings indicate that ScoC functions as a transcription factor with strain-specific effects, playing a positive role in flagella formation and motility in B. pumilus, which contrasts with its role in B. subtilis.

Article Abstract

Background: Bacillus pumilus is a Gram-positive and endospore-forming bacterium broadly existing in a variety of environmental niches. Because it produces and secrets many industrially useful enzymes, a lot of studies have been done to understand the underlying mechanisms. Among them, scoC was originally identified as a pleiotropic transcription factor negatively regulating protease production and sporulation in B. subtilis. Nevertheless, its role in B. pumilus largely remains unknown.

Results: In this study we successfully disrupted scoC gene in B. pumilus BA06 and found increased total extracellular protease activity in scoC mutant strain. Surprisingly, we also found that scoC disruption reduced cell motility possibly by affecting flagella formation. To better understand the underlying mechanism, we performed transcriptome analysis with RNA sequencing. The result showed that more than one thousand genes were alternated at transcriptional level across multiple growth phases, and among them the largest number of differentially expressed genes (DEGs) were identified at the transition time point (12 h) between the exponential growth and the stationary growth phases. In accordance with the altered phenotype, many protease genes especially the aprE gene encoding alkaline protease were transcriptionally regulated. In contrast to the finding in B. subtilis, the aprN gene encoding neutral protease was transcriptionally downregulated in B. pumilus, implicating that scoC plays strain-specific roles.

Conclusions: The pleiotropic transcription factor ScoC plays multiple roles in various cellular processes in B. pumilus, some of which were previously reported in B. subtilis. The supervising finding is the identification of ScoC as a positive regulator for flagella formation and bacterial motility. Our transcriptome data may provide hints to understand the underlying mechanism.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6492404PMC
http://dx.doi.org/10.1186/s12864-019-5671-8DOI Listing

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