Dissemination of NDM-1 carbapenemase-producer Providencia stuartii strains in Romanian hospitals: a multicentre study.

J Hosp Infect

Tîrgu-Mures County Emergency Clinical Hospital, Romania; University of Medicine, Pharmacy, Science and Technology of Tîrgu-Mureş, Romania.

Published: October 2019

Several Romanian hospitals have noted increasing isolation of Providencia stuartii strains in recent years, with an alarming rate of carbapenem resistance. In order to provide molecular epidemiological data regarding their dissemination, 77 P. stuartii strains collected from five hospitals located in different regions of Romania were analysed. All strains harboured IncA/C plasmid, and 67 carried the blaNDM-1 gene. Six clonal clusters were differentiated by pulsed-field gel electrophoresis. The predominant subtype was found in all five hospitals. Our study highlights the need for efficient infection-control measures, the optimization of antibiotic use and the targeted surveillance for carbapenemase-producing P. stuartii.

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http://dx.doi.org/10.1016/j.jhin.2019.04.015DOI Listing

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  • Providencia stuartii is a rising cause of hospital infections, and a multidrug-resistant strain (MF1) was discovered in swine wastewater.
  • The whole genome sequencing of MF1 revealed numerous antibiotic resistance genes, including a new transposon (Tn6450M) related to known resistance elements.
  • Tn6450M is stably integrated into the MF1 genome and carries genes associated with resistance to third-generation cephalosporins, raising concerns about the potential spread of antibiotic resistance through intergeneric transmission.
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