Objective: This study aims to explore the effect of triggering a receptor expressed on myeloid cells 2 (TREM2) in lipopolysaccharide (LPS)-stimulated primary microglia cells and its mechanisms.
Methods: We isolated mouse primary microglia cells in this study. The levels of TREM2, IL-6, and TNF-α in peripheral blood of SCI patients were examined by ELISA assay. The qRT-PCR and western blotting assays were performed to investigate the effects of TREM2 in LPS-induced primary microglia cells.
Results: The levels of TREM2, IL-6, and TNF-α significantly increased in peripheral blood of SCI patients and in LPS-stimulated primary microglia cells. Down-regulation of TREM2 significantly reduced the expression of p-NF-κB and the production of IL-6 and TNF-α. In addition, PDTC (a NF-κB inhibitor) treatment inhibited TREM2 overexpression induced-production of IL-6 and TNF-α.
Conclusions: Downregulating the expression of TREM2 can inhibit the release of inflammatory factors from LPS-stimulated microglia by inhibiting NF-κB signaling pathway activity, which may be beneficial for the treatment of SCI.
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