AI Article Synopsis
- NLR immune receptors are crucial for plant immunity, activating in response to pathogen effectors, with autoactive NLRs providing a broader resistance without needing these effectors.
- * In this study, specific genes from dicots were found to be autoactive in monocots, enabling them to resist various fungal and bacterial pathogens, as well as pests like the brown planthopper.
- * Transgenic plants with these autoactive NLRs showed strong immune responses, including increased reactive oxygen species and defense gene expression, highlighting their potential for improving crop resistance through breeding.
Article Abstract
The nucleotide-binding domain leucine-rich repeat (NLR) immune receptors play important roles in innate plant immunity. The activation of NLRs is specifically induced by their cognate effectors released from pathogens. Autoactive NLRs are expected to confer broad-spectrum resistance because they do not need cognate effectors to activate their immune responses. In this study, we demonstrated that the genes and () from were autoactive in and conferred broad-spectrum resistance to fungal pathogen , bacterial pathogen (), and pest brown planthopper (BPH, Stål). These results revealed that interfamily transfer of dicot NLRs to monocot species could be functional. The transgenic plants displayed early and strong induction of reactive oxygen species (ROS), callose deposition, and expression of defense-related genes after challenged with . The transcriptome analysis showed that the expressions of some defense-related genes were primed to adapt the transformed autoactive NLRs in the transgenic plants. This study indicates that autoactive NLRs are a promising resource for breeding crops with broad-spectrum resistance and provides new insights for engineering disease resistance.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6459959 | PMC |
| http://dx.doi.org/10.3389/fpls.2019.00417 | DOI Listing |
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