The filamentous fungus was recently used as a heterologous host for fungal secondary metabolite production. Here, we aimed to produce the plant polyketide curcumin in . Curcumin is synthesized from feruloyl-coenzyme A (CoA) and malonyl-CoA by curcuminoid synthase (CUS). expressing produced curcumin (64 μg/plate) on an agar medium containing feruloyl--acetylcysteamine (a feruloyl-CoA analog). To increase curcumin yield, we attempted to strengthen the supply of malonyl-CoA using two approaches: enhancement of the reaction catalyzed by acetyl-CoA carboxylase (ACC), which produces malonyl-CoA from acetyl-CoA, and inactivation of the acetyl-CoA-consuming sterol biosynthesis pathway. Finally, we succeeded in increasing curcumin yield sixfold by the double disruption of and ; SnfA is a homolog of SNF1, which inhibits ACC activity by phosphorylation in and SCAP is positively related to sterol biosynthesis in . This study provided useful information for heterologous polyketide production in .
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